Buffer solution can control the porosity of DNA-chitosan complexes
Buffer solution can control the porosity of DNA-chitosan complexes
复制标题
DOI：
10.1002/jbm.b.30334
复制
发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
Okahata, Y
中科院分区：
文献类型：
--
作者：
Fukushima, T;Hayakawa, T;Okahata, Y
目前的研究检查了 DNA-壳聚糖复合物的孔特性和生物效应，该复合物可能可用作组织工程的支架。 DNA-壳聚糖复合物的孔隙率通过用几种不同的 pH 7.2 缓冲溶液冲洗来控制，包括磷酸盐缓冲盐水 (PBS)、Tris-HCl、硼酸和 N-(2 羟乙基)哌嗪-N'-(2-乙磺酸) (HEPES)。用 PBS 冲洗产生 84% 的孔隙率，而用 Tris-HCl 冲洗产生 94% 的孔隙率。进一步发现，盐酸柔红霉素复合物嵌入并结合在DNA-壳聚糖复合物的凹槽中，表明复合物中的DNA保持了其双链螺旋结构。 DNA-壳聚糖复合物对 MG-63 成骨细胞样细胞没有毒性，并且当皮下植入大鼠背部时仅引起轻微的组织反应。这些结果表明缓冲液冲洗的 DNA-壳聚糖复合物可用作组织工程中的支架材料。 (c) 2005 年 Wiley 期刊公司。
The current studies examine the pore properties and biological effects of DNA-chitosan complexes, which may be useful as scaffolds for tissue engineering. The porosity of the DNA-chitosan complexes was controlled by rinsing them with several different pH 7.2 buffer solutions, including phosphate-buffered saline (PBS), Tris-HCI, boric acid, and N-(2hydroxyethyl)piperazine-N'-(2-ethanesufonic acid) (HEPES). Rinsing with PBS resulted in 84% porosity, whereas rinsing with Tris-HCI produced 94% porosity. It was further found that daunorubicin hydrochloride complex intercalated with and bound to the groove of the DNA-chitosan complexes, indicating that DNA in the complexes maintains its double-stranded helical structure. The DNA-chitosan complexes were not toxic to MG-63 osteoblast-like cells and caused only a mild tissue response when implanted subcutaneously in the backs of rats. These results suggest that buffer-rinsed DNA-chitosan complexes may be useful as a scaffold material in tissue engineering. (c) 2005 Wiley Periodicals, Inc.