Access to the maize genome: An integrated physical and genetic map
Access to the maize genome: An integrated physical and genetic map
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DOI：
10.1104/pp.010953
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发表时间：
2002-01-01
期刊：
影响因子：
7.4
通讯作者：
Wing, R
中科院分区：
文献类型：
--
作者：
Coe, E;Cone, K;Wing, R
作物植物研究将取得革命性的进展，包括：根据其在基因组中的功能和/或位置克隆目标基因；记录所有基因及其相互作用；定义和探索物种中所有现有的遗传多样性；利用密切相关物种中基因的功能信息和共线关系来推断作物的基因功能。然而，挑战是开发一套全面和系统的资源来促进这些研究努力。玉米基因组资源(Zea Mays)将支持玉米基因组测序，并将补充和促进谷物、其他牧草和其他农作物的研究。对玉米来说，开发基因组学工具意味着面对规模和复杂性的严峻现实。在大约2,500兆碱基的情况下，玉米基因组的大小与人类相当，而且由于多个重复元素家族的丰富，其复杂性可能会更大。因此，获取玉米基因组的最好办法是听从歌德的建议，通过深入了解它的各个部分来寻求进入整体：Willst du ins Unendliche schreiten，Geh Nur im Endlichen nach Allen Seiten。-约翰·沃尔夫冈·冯·歌德要有效地将基因组拆分并重新组合在一起，需要结合遗传和物理作图。原则上，基因图谱通过交叉和重组部分来对基因组进行细分和排序，而物理图谱通过确定基因组片段(部分)之间的重叠来对基因组片段(部分)进行排序。因此，
Crop plant research is poised to make revolutionary strides including the following: cloning target genes based on their function and/or their position in the genome; documenting all genes and their interplay; defining and exploring all the existing genetic diversity in a species; and using functional information and syntenic relationships of genes in closely related species to extrapolate gene function in crop plants. The challenge, however, is to develop a set of comprehensive and systematic resources to facilitate these research endeavors. Genomic resources in maize (Zea mays) will undergird sequencing of the maize genome and will complement and contribute to research in the cereals, other grasses, and other crop plants.For maize, developing genomics tools means facing the daunting realities of size and complexity. At approximately 2,500 megabases, the maize genome is comparable in size to that of humans, and the complexity is likely to be greater because of the abundance of multiple families of repetitive elements. Thus, gaining access to the maize genome is best tackled by following Goethe’s advice to seek entry to the whole by going to its parts: Willst du ins Unendliche schreiten, Geh nur im Endlichen nach allen Seiten.—Johann Wolfgang von Goethe To efficiently take a genome apart and put it back together requires a combination of genetic and physical mapping. In principle, genetic mapping serves to subdivide and order the genome by crossing over and recombining parts, whereas physical mapping allows ordering of genomic fragments (parts) by determining the overlap among them. Accordingly, the