Analysis of population structure and insecticide resistance in mosquitoes of the genus Culex, Anopheles and Aedes from different environments of Greece with a history of mosquito borne disease transmission
Analysis of population structure and insecticide resistance in mosquitoes of the genus Culex, Anopheles and Aedes from different environments of Greece with a history of mosquito borne disease transmission
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DOI：
10.1016/j.actatropica.2017.06.005
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发表时间：
2017-10-01
期刊：
影响因子：
2.7
通讯作者：
Vontas, John
中科院分区：
文献类型：
--
作者：
Fotakis, Emmanouil A.;Chaskopoulou, Alexandra;Vontas, John
希腊最近受到几种蚊媒疾病的影响，2010年爆发的西尼罗河病毒（WNV）是欧洲报告的最大的疾病之一。目前，在经济和难民危机的中心，每年有1 600多万游客来访，蚊子传播疾病的综合管理是公共卫生和经济的优先事项。媒介生物控制主要依靠杀虫剂，但杀虫剂抗性和蚊子区系的数据是成功应用的关键。我们确定了媒介生物传播疾病脆弱性增加地区的蚊子种类组成和种群动态，并调查了主要滋扰和疾病媒介对杀虫剂的抗性状况。塞萨洛尼基和埃夫罗斯的蚊子密度很高，其中最流行的物种包括令人讨厌的物种-卡斯皮乌斯伊蚊、已知的西尼罗河病毒病媒-尖音库蚊和潜在的疟疾病媒-赫坎按蚊。这两种病媒物种的数量都在夏末达到高峰。白纹伊蚊在塞萨洛尼基的记录密度很高，但在埃夫罗斯没有。值得注意的是，CX。在塞萨洛尼基和阿提卡记录了相当数量的pipiens杂交种，它们表现出机会性的叮咬行为，并被怀疑与西尼罗河病毒的传播有关。尖音库蚊（Culex pipiens）和安氏按蚊（An. hyrcanus，但不是Ae。卡斯皮乌斯蚊对溴氰菊酯表现出中等水平的抗性。在没有接触病媒控制的地区出现抗药性表明，其他因素也可能选择抗药性，即农业中的农药应用。在Cx中检测到L1014 F和L101 C kdr突变。pipiens populations.赫坎按蚊的耐药性与L1014位点的突变无关。Ace-1突变，赋予不敏感的有机磷和氨基甲酸酯检测在所有Cx的低频率。pipiens populations. Cx中P450和酯酶活性增加。pipiens个体从塞萨洛尼基。我们的研究为可持续和有效的病媒控制战略和预防疾病暴发提供了证据。
Greece has been recently affected by several mosquito borne diseases with the West Nile Virus (WNV) outbreak in 2010 being one of the largest reported in Europe. Currently at the epicenter of an economic and refugee crisis and visited by over 16 million tourists a year the integrated management of diseases transmitted by mosquitoes is a public health and economic priority. Vector control programs rely mainly on insecticides, however data on insecticide resistance and the mosquito fauna is essential for successful applications.We determined the mosquito species composition and population dynamics in areas of increased vulnerability to vector borne disease transmission, as well as investigated the resistance status of major nuisance and disease vectors to insecticides. High mosquito densities were recorded in Thessaloniki and Evros, with Aedes caspius, a nuisance species, Culex pipiens, a known vector of WNV and Anopheles hyrcanus a potential vector of malaria being among the most prevalent species. Both vector species populations reached their peak in late summer. Aedes albopictus was recorded at high densities in Thessaloniki, but not in Evros. Notably, Cx. pipiens hybrids, which show an opportunistic biting behavior and are suspected to be involved in the transmission of the WNV, were recorded in considerable numbers in Thessaloniki and Attica. Culex pipiens and An. hyrcanus, but not Ae. caspius mosquitoes, showed moderate levels of resistance to deltamethrin. The presence of resistance in areas not exposed to vector control indicates that other factors could be selecting for resistance, i.e. pesticide applications for agriculture. Both L1014F and L101C kdr mutations were detected in Cx. pipiens populations. Anopheles hyrcanus resistance was not associated with mutations at the L1014 site. The Ace-1 mutations conferring insensitivity to organophosphates and carbamates were detected at low frequencies in all Cx. pipiens populations. Increased activity of P450s and esterases was found in Cx. pipiens individuals from Thessaloniki. Our study contributes evidence for sustainable and efficient vector control strategies and the prevention of disease outbreaks.