Effect of acetic acid bacteria colonization on oviposition and feeding site choice in Drosophila suzukii and its related species
Effect of acetic acid bacteria colonization on oviposition and feeding site choice in Drosophila suzukii and its related species
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DOI：
10.1101/2023.03.20.533419
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发表时间：
2023-03
期刊：
影响因子：
--
通讯作者：
Airi Sato;J. Yew;Aya Takahashi
中科院分区：
文献类型：
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作者：
Airi Sato;J. Yew;Aya Takahashi
产卵地点的选择对后代的表现有很大的影响。与其他寄生在腐烂水果上的醋蝇不同，铃木果蝇通过使用它们巨大的锯齿状产卵器(卵子)将卵产卵到硬熟的水果中。与其他物种相比，这种行为具有优势，因为它能更早地获得寄主果实，并避免竞争。然而，幼虫并不完全适应低蛋白质的饮食，完整的健康水果的供应受到季节性限制。因此，为了研究该物种的产卵地点对微生物生长的偏好，我们用单种共生果蝇醋酸菌、醋酸杆菌和葡萄糖杆菌进行了产卵试验。对有或没有细菌生长的媒介的产卵地点偏好进行了量化，包括多个铃鹿及其近缘种--亚孢子虫和双肢杆菌，以及一种典型的发酵水果消费者--黑腹夜蛾。我们的比较表明，无论是在物种内部还是跨物种，醋酸菌的生长部位都有持续的偏好程度，这表明生态位分离是显著的，但不是完全的。对葡萄糖杆菌的偏好在不同重复之间有很大的差异，而菌株之间没有明显的差异。此外，含有醋酸杆菌的媒介的取食地点的种间差异表明，种间的产卵地点选择的差异与取食地点的选择无关。我们的产卵测定表明，每种果蝇的多个品系对醋酸菌生长的偏好揭示了这些果蝇之间共享资源的内在特性。
Oviposition site choice has a large impact on offspring performance. Unlike other vinegar flies that colonize decaying fruits, Drosophila suzukii lay eggs into hard ripening fruits by using their enlarged and serrated ovipositors (oviscapts). This behavior has an advantage over other species by providing access to the host fruit earlier and avoiding competition. However, the larvae are not fully adapted to a low-protein diet, and the availability of intact healthy fruits is seasonally restricted. Thus, to investigate oviposition site preference for microbial growth in this species, we conducted an oviposition assay using single species of commensal Drosophila acetic acid bacteria, Acetobacter and Gluconobacter. The oviposition site preferences for media with or without bacterial growth were quantified in multiple strains of D. suzukii and its closely related species, D. subpulchrella and D. biarmipes, and a typical fermenting-fruit consumer, D. melanogaster. Our comparisons demonstrated a continuous degree of preference for sites with Acetobacter growth both within and across species, suggesting that the niche separation is notable but not complete. The preference for Gluconobacter showed large variations among replicates and no clear differences between the strains. In addition, the lack of interspecific differences in feeding site preference for Acetobacter-containing media implies that the interspecific divergence in oviposition site preference occurred independently from the feeding site preference. Our oviposition assays measuring the preference of multiple strains from each fly species for acetic acid bacteria growth revealed intrinsic properties of shared resource usage among these fruit fly species.