Natural co-occurrence of aflatoxin B1, fumonisin B1 and ochratoxin A in barley and corn foods from Korea
Natural co-occurrence of aflatoxin B1, fumonisin B1 and ochratoxin A in barley and corn foods from Korea
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DOI：
10.1080/02652030210151840
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发表时间：
2002-11-01
影响因子：
2.9
通讯作者：
Kim, YB
中科院分区：
文献类型：
--
作者：
Park, JW;Kim, EK;Kim, YB
对1998-99年在韩国首尔随机采集的127个样品进行了黄曲霉毒素B-1（AF B（1））、伏马菌素B-1和赭曲霉毒素A（OTA）的调查，这些样品包括30个食品级大麦、32个大麦食品、18个食品级玉米和47个玉米食品。真菌毒素的存在进行了分析，直接竞争酶联免疫吸附试验（ELISA），并确认使用高效液相色谱法（HPLC）从ELISA的阳性样品。ELISA法对10 ng g（-1）AFB（1）、OTA和50 ng g（-1）FB（1）的回收率分别为106%、87%和105%，而HPLC法的回收率分别为80%、79%和84%。ELISA法对AFB（1）、FB 1和OTA的检测限分别为1、5和5 ng g（-1），HPLC法的检测限分别为0.6、35和1 ng g（-1）。在32份大麦食品样品中发现4份（12%）、2份（6%）和4份（12%）天然存在的黄曲霉毒素B1、FB 1和OTA，平均含量分别为26、16和9 ng g（-1）。在47份玉米食品中分别有4份（8%）和9份（19%）检出黄曲霉毒素B1和FB 1，平均值分别为20和74 ng g（-1），而在所有玉米食品中均未检出OTA。在所有食品级大麦和玉米样品中均未检测到黄曲霉毒素B1、FB 1和OTA。这是韩国首次报道大麦和玉米食品中AFB（1）和FB 1的自然共存以及OTA的监测。
A survey for aflatoxin B-1 (AFB(1)), fumonisin B-1 and ochratoxin A (OTA) was conducted on 127 samples that included 30 food-grade barley, 32 barley foods, 18 food-grade corn and 47 corn foods, randomly collected during 1998-99 in Seoul, Korea. The presence of mycotoxins was analysed by direct competitive enzyme-linked immunosorbent assay (ELISA), and most of the positive samples from ELISA were confirmed using high-performance liquid chromatography (HPLC). Recoveries of AFB(1) and OTA spiked at 10 ng g(-1) and FB1 spiked at 50 ng g(-1) were 106, 87 and 105% by ELISA, whereas those by HPLC were 80, 79 and 84%, respectively. Detection limits by ELISA for AFB(1), FB1 and OTA were 1, 5 and 5 ng g(-1), and those by HPLC were 0.6, 35 and 1 ng g(-1). Naturally occurring AFB(1), FB1 and OTA were found in 4/32 (12%), 2/32 (6%) and 4/32 (12%) samples of barley foods with an average of 26, 16 and 9 ng g(-1), respectively. AFB(1) and FB1 in corn foods were detected in 4/47 (8%) and 9/47 (19%) samples with the average being 20 and 74 ng g(-1) while no OTA was found in any corn foods samples. No AFB(1), FB1 or OTA was detected in any of food-grade barley and corn samples. This is the first report on the natural co-occurrence of AFB(1) and FB1 in barley and corn foods as well as on surveillance of OTA in Korea.