Biological effect of radiation‐degraded alginate on flower plants in tissue culture
Biological effect of radiation‐degraded alginate on flower plants in tissue culture
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辐射降解海藻酸盐对组织培养花卉植物的生物学效应
DOI：
10.1042/ba20030058
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发表时间：
2003
影响因子：
2.8
通讯作者：
T. Nakanishi
中科院分区：
文献类型：
--
作者：
L. Luan;N. Q. Hien;N. Nagasawa;T. Kume;F. Yoshii;T. Nakanishi
重均分子质量(MW)约为照射剂量为9.04×105Da，照射剂量为10~200kGy4%(w/v)水溶液。利用降解后的海藻酸盐产品研究了其在组织培养中作为植物生长促进剂的效果。海藻酸盐在75kGy处照射，相对分子质量约。1.43×104Da对三种花卉植物生长的正效应最大，分别为柠檬、石蒜花和菊花。用30~200 mg/L的辐照海藻酸盐处理植株，芽增殖率由对照的17.5%提高到40.5%。在试管苗培养中，添加100 mg/L辐照海藻酸盐的菊花、藤本植物和柠檬的地上部高度(9.7-23.2%)、根长(9.7-39.4%)和鲜重(8.1-19.4%)均比对照增加。在温室条件下，经辐照海藻酸盐处理的移栽花卉幼苗的成活率与对照基本相同。然而，处理的试管苗获得了更好的生长。
Alginate with a weight‐average molecular mass (Mw) of approx. 9.04×105 Da was irradiated at 10–200 kGy in 4% (w/v) aqueous solution. The degraded alginate product was used to study its effectiveness as a growth promoter for plants in tissue culture. Alginate irradiated at 75 kGy with an Mw of approx. 1.43×104 Da had the highest positive effect in the growth of flower plants, namely limonium, lisianthus and chrysanthemum. Treatment of plants with irradiated alginate at concentrations of 30–200 mg/l increased the shoot multiplication rate from 17.5 to 40.5% compared with control. In plantlet culture, 100 mg/l irradiated alginate supplementation enhanced shoot height (9.7–23.2%), root length (9.7–39.4%) and fresh biomass (8.1–19.4%) of chrysanthemum, lisianthus and limonium compared with that of the untreated control. The survival ratios of the transferred flower plantlets treated with irradiated alginate were almost the same as the control value under greenhouse conditions. However, better growth was attained for the treated plantlets.