Reversine, a substituted purine, exerts an inhibitive effect on human renal carcinoma cells via induction of cell apoptosis and polyploidy
Reversine, a substituted purine, exerts an inhibitive effect on human renal carcinoma cells via induction of cell apoptosis and polyploidy
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Reversine 是一种取代嘌呤，通过诱导细胞凋亡和多倍体对人肾癌细胞发挥抑制作用
DOI：
10.2147/ott.s158198
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发表时间：
2018-01-01
影响因子：
4
通讯作者：
Lin, Jian
中科院分区：
文献类型：
--
作者：
Cheng, Li;Wang, Hao;Lin, Jian
研究背景肾细胞癌（renal cell carcinoma，RCC）是发生于肾上皮的最常见的肾癌。高达33.3%的RCC患者接受局部肿瘤切除术治疗后会出现复发或转移。因此，迫切需要优化的治疗方案，以改善肾癌的预后。逆转录酶最近被报道在癌症治疗中发挥关键作用。材料与方法本研究评价了逆转录酶对786-O和ACHN细胞系的细胞活力、集落形成、凋亡和细胞周期的影响。结果逆转录酶对两种细胞株的增殖均有明显的抑制作用，并呈时间和剂量依赖性。在高浓度的逆转录酶处理下观察到多倍体形成。此外，reversine通过caspase依赖的凋亡途径诱导细胞死亡，这可以被泛caspase抑制剂Z-VAD-FMK部分抑制。结论Reversine能有效抑制肾癌细胞的增殖，有望成为肾癌治疗的新方案。
Background Human renal cell carcinoma (RCC) is the most common type of kidney cancer that arises from the renal epithelium. Up to 33.3% of RCC patients treated with local tumor resections will subsequently develop recurrence or metastases. Thus, optimized therapeutic regimes are urgently needed to improve the prognosis of RCC. Reversine was recently reported to exert critical roles in cancer therapy. Materials and methods This study evaluated the anti-tumor effects of reversine on cell viability, colony formation, apoptosis, and cell cycle in 786-O and ACHN cell lines. Results It was demonstrated that reversine significantly inhibited the proliferation of both cell lines in time- and dose-dependent manners. Polyploidy formation was observed under high-concentration reversine treatment. In addition, reversine induced cell death via caspase-dependent apoptotic pathways, which could be partially inhibited by Z-VAD-FMK, a pan-caspase inhibitor. Conclusion Reversine could effectively suppress the proliferation of human RCC cells, and may serve as a novel therapeutic regimen for RCC in clinical practice.