Presence of less heavily encapsulated Klebsiella pneumoniae capsular type 1 in semen of healthy stallions and cervical swabs of mares suffering from metritis and comparison of virulence between heavily and less heavily encapsulated strains.
Presence of less heavily encapsulated Klebsiella pneumoniae capsular type 1 in semen of healthy stallions and cervical swabs of mares suffering from metritis and comparison of virulence between heavily and less heavily encapsulated strains.
复制标题
健康公马的精液和患有子宫炎的母马的宫颈拭子中是否存在包膜较轻的 1 型肺炎克雷伯菌，以及重包膜菌株和包膜较轻菌株之间的毒力比较。
DOI：
10.1292/jvms1939.50.313
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
R. Yanagawa
中科院分区：
文献类型：
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作者：
N. Kikuchi;N. Takayanagi;Y. Kosaka;T. Hiramune;R. Yanagawa
从健康公马的精液和患有子宫炎的母马的宫颈拭子中分离出1型荚膜较轻的肺炎克雷伯菌（K.pneumoniae）菌株，该菌株与普通的重包膜菌株不同。包膜较轻的菌株，其蒴果横径为1.3-2.2μm，明显小于包膜较重的菌株，其蒴果直径大于3.0μm。从健康公马精液中分离到的 5 株肺炎克雷伯菌 1 型荚膜，其中 1 株（20.0%）为重度包膜，其余 4 株（80.0%）为轻度包膜；而从患有子宫炎的母马宫颈拭子中分离到 83 株肺炎克雷伯菌 1 型，其中 76 株（91.6%）为重度包膜，仅 7 株为重度包膜。 (8.4%) 的封装程度较低。包膜较轻的菌株对小鼠的毒力较低（精液来源的菌株，LD50为10至10 CFU）或中等（宫颈拭子来源的菌株，LD50为10 CFU），很容易被马和鼠多形核白细胞（PMNL）吞噬，而包膜较重的菌株则表现出高毒力（LD50为102至104） CFU）用于小鼠，并受到马和鼠 PMNL 的吞噬作用的保护。
Less heavily encapsulated Klebsiella pneumoniae (K.pneumoniae) strains of capsular type 1, which were different from ordinary heavily encapsulated strains, were isolated from semen of healthy stallions and from cervical swabs of mares suffering from metritis. Less heavily encapsulated strains possessed capsules ranging 1.3-2.2μm in transverse diameter, which were obviously smaller than those of heavily encapsulated strains, which possessed capsules more than 3.0μm in diameter. Of the 5 strains of K.pneumoniae capsular type 1 isolated from the semen of healthy stallions, 1 (20.0%) was heavily encapsulated and the remaining 4 (80.0%) were less heavily encapsulated, while of the 83 strains of K.pneumoniae capsular type 1 from the cervical swabs of mares suffering from metritis, 76 (91.6%) were heavily encapsulated and only 7 (8.4%) were less heavily encapsulated. Less heavily encapsulated strains showed low (semen-origin strains, LD50 was 10 to 10 CFU) or intermediate (cervical swab-origin strain, LD50 was 10 CFU) virulence for mice and were easily phagocytized by equine and murine polymorphonuclear leukocytes (PMNL), while heavily encapsulated strains showed high virulence (LD50 was 102 to 104 CFU) for mice and were protected from phagocytosis by the equine and murine PMNL.