In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide
In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide
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DOI：
10.1016/j.jbiosc.2012.03.020
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发表时间：
2012-08-01
影响因子：
2.8
通讯作者：
Niwano, Yoshimi
中科院分区：
文献类型：
--
作者：
Hayashi, Eisei;Mokudai, Takayuki;Niwano, Yoshimi
本研究旨在评价本实验室开发的一种用于治疗牙周炎等口腔感染性疾病的新型消毒体系--H2 O2光解羟基自由基产生体系的体内外抗菌活性。首先，通过应用电子自旋共振-自旋捕获技术，证实了在400和465 nm波长下用双波长发光二极管（LED）照射1 mol I-1 H2 O2的H2 O2光解产生羟基自由基。其次，在类似的条件下检测了该系统的杀菌效果，其中用LED光照射悬浮在1 mol I-1 H2 O2中的金黄色葡萄球菌，导致细菌的殖民地形成单位（CFU）在2 min的短时间内显著减少。通过培养研究评价金黄色葡萄球菌感染。由于回收的S.金黄色葡萄球菌的体外抗菌作用，预期H2 O2光解产生的羟基自由基的体外抗菌作用可以很好地反映在体内浅表细菌感染中。(c)2012年，生物技术学会，日本。All rights reserved.
The present study aimed to evaluate in vitro and in vivo antibacterial activity of hydroxyl radical generation system by photolysis of H2O2, which is a new disinfection system for the treatment of oral infection diseases such as periodontitis developed in our laboratory. Firstly, generation of the hydroxyl radical by the photolysis of H2O2 in which 1 mol I-1 H2O2 was irradiated with a dual wavelength-light emitting diode (LED) at wavelengths of 400 and 465 nm was confirmed by applying an electron spin resonance-spin trapping technique. Secondly, the bactericidal effect of the system was examined under a similar condition in which Staphylococcus aureus suspended in 1 mol I-1 H2O2 was irradiated with LED light, resulting in substantial reduction of the colony forming unit (CFU) of the bacteria within a short time as 2 min. Finally, in vivo antibacterial effect of the photolysis of H2O2 on a rat model of S. aureus infection was evaluated by a culture study. Since a significant reduction of recovered CFU of S. aureus was obtained, it is expected that in vitro antibacterial effect attributable to hydroxyl radicals generated by photolysis of H2O2 could be well reflected in in vivo superficial bacterial infection. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.