Hydrogen Sulfide Alleviates Postharvest Senescence of Grape by Modulating the Antioxidant Defenses.
Hydrogen Sulfide Alleviates Postharvest Senescence of Grape by Modulating the Antioxidant Defenses.
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硫化氢通过调节抗氧化防御来减轻葡萄采后衰老
DOI：
10.1155/2016/4715651
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发表时间：
2016
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Ni ZJ;Hu KD;Song CB;Ma RH;Li ZR;Zheng JL;Fu LH;Wei ZJ;Zhang H
硫化氢 (H2S) 已被确定为植物中重要的气体信号。在这里，我们研究了H2S减轻巨峰葡萄采后衰老和腐烂的机制。外源施用1.0mM NaHS释放的H2S显着降低了葡萄浆果的腐烂和脱粒率。施用H2S还可以防止葡萄串的重量损失，并抑制采后储存期间葡萄果肉的硬度、可溶性固形物和可滴定酸度的下降。叶绿素和类胡萝卜素含量数据表明，H2S 在防止叶绿素分解和类胡萝卜素在葡萄轴和果肉中积累方面发挥着作用。与水对照相比，施用外源H2S使葡萄浆中抗坏血酸、类黄酮、总酚、还原糖和可溶性蛋白质的含量保持显着较高的水平。同时，H2S显着减少了葡萄浆中丙二醛（MDA）、过氧化氢（H2O2）和超氧阴离子（）的积累。进一步的研究表明，H2S 增强了葡萄皮和果肉中抗氧化酶抗坏血酸过氧化物酶 (APX) 和过氧化氢酶 (CAT) 的活性，并降低了脂氧合酶 (LOX) 的活性。总而言之，我们提供了强有力的证据，证明硫化氢通过调节抗氧化酶和减弱脂质过氧化作用，有效缓解巨峰葡萄采后衰老和腐烂。
Hydrogen sulfide (H2S) has been identified as an important gaseous signal in plants. Here, we investigated the mechanism of H2S in alleviating postharvest senescence and rotting of Kyoho grape. Exogenous application of H2S released from 1.0 mM NaHS remarkably decreased the rotting and threshing rate of grape berries. H2S application also prevented the weight loss in grape clusters and inhibited the decreases in firmness, soluble solids, and titratable acidity in grape pulp during postharvest storage. The data of chlorophyll and carotenoid content suggested the role of H2S in preventing chlorophyll breakdown and carotenoid accumulation in both grape rachis and pulp. In comparison to water control, exogenous H2S application maintained significantly higher levels of ascorbic acid and flavonoid and total phenolics and reducing sugar and soluble protein in grape pulp. Meanwhile, H2S significantly reduced the accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion () in grape pulp. Further investigations showed that H2S enhanced the activities of antioxidant enzymes ascorbate peroxidase (APX) and catalase (CAT) and decreased those of lipoxygenase (LOX) in both grape peels and pulp. In all, we provided strong evidence that H2S effectively alleviated postharvest senescence and rotting of Kyoho grape by modulating antioxidant enzymes and attenuating lipid peroxidation.