Inhibitor of vesicular stomatitis virus transcriptase in purified virions
Inhibitor of vesicular stomatitis virus transcriptase in purified virions
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纯化病毒颗粒中水泡性口炎病毒转录酶的抑制剂
DOI：
10.1038/248045a0
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发表时间：
1974
期刊：
影响因子：
64.8
通讯作者：
D. Kingsbury
中科院分区：
文献类型：
--
作者：
J. Perrault;D. Kingsbury
水泡性口炎病毒（VSV）颗粒含有RNA依赖性RNA聚合酶，其在体外产生与体内mRNA大小和极性相同的RNA分子1 -3。尚不清楚哪种结构蛋白代表酶活性。在病毒体中只检测到五种蛋白质（L、G、N、MS和M，可能还有一些其他的次要成分）4，几项研究表明，G蛋白（糖蛋白表面刺突）和M蛋白（膜蛋白），甚至相关的膜脂质都不是转录酶活性所必需的5 -7。
VESICULAR stomatitis virus (VSV) particles contain an RNA-dependent RNA polymerase which gives rise in vitro to RNA molecules of the same size and polarity as the mRNA found in vivo1–3. It is not clear which structural protein(s) represents the enzymatic activity. Only five proteins (L, G, N, MS, and M, with possibly some additional minor constituents) are detected in the virions4, and several studies have suggested that neither the G protein (glycoprotein surface spike) nor the M protein (membrane protein), nor even associated membrane lipids are necessary for transcriptase activity5–7.