Rapid, concurrent analysis of dopamine, 5-hydroxytryptamine, their precursors and metabolites utilizing high performance liquid chromatography with electrochemical detection: analysis of brain tissue and cerebrospinal fluid.
Rapid, concurrent analysis of dopamine, 5-hydroxytryptamine, their precursors and metabolites utilizing high performance liquid chromatography with electrochemical detection: analysis of brain tissue and cerebrospinal fluid.
复制标题
利用高效液相色谱和电化学检测对多巴胺、5-羟色胺、其前体和代谢物进行快速、同步分析：脑组织和脑脊液分析。
DOI：
10.1016/0024-3205(82)90675-0
复制
发表时间：
1982
期刊：
影响因子：
6.1
通讯作者：
Johnston,CA
中科院分区：
文献类型：
--
作者：
Nielsen,JA;Johnston,CA
Assays capable of measuring picomole quantities of dopamine (DA), 5-hydroxytryptamine (5-HT), several of their precursors and metabolites concurrently within 25 minutes were developed utilizing high performance liquid chromatography with electrochemical detection (LCEC). Several parameters of the LCEC were altered in order to separate the compounds while maintaining a short assay time. The final LCEC systems demonstrated biological utility in that the DA metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), and the 5-HT metabolite 5-hydroxy-3-indoleacetic acid (5-HIAA) were detected in rat cerebrospinal fluid; in addition to these compounds, DA and 5-HT were measurable in the striatum, hypothalamus and median eminence of the rat brain. Pargyline decreased the concentrations of DOPAC, HVA and 5-HIAA and increased the 5-HT concentration in all three brain regions, and increased the DA concentration in the striatum. Probenecid increased all three acid metabolite concentrations in the hypothalamus and median eminence, while only the HVA and 5-HIAA concentrations were increased in the striatum. The DA and 5-HT concentrations were unaltered. The LCEC methods described in this paper should be useful in elucidating the mechanisms and roles of 5-HT and DA neurons in experimental paradigms of biological interest.
登录
查看更多内容
影响因子：
6.1
作者：
W. Lyness;N. M. Friedle;K. E. Moore
通讯作者：
K. E. Moore
高效液相色谱电化学检测同时测定 5-羟基色氨酸和 L-二羟基苯丙氨酸。
DOI：
10.1016/s0378-4347(00)80425-9
发表时间：
1982
期刊：
Journal of chromatography
影响因子：
--
作者：
A. Shum;M. Sole;G. van Loon
通讯作者：
G. van Loon
通过推拉插管持续灌注大鼠大脑中 3H-多巴胺的代谢：安非他明或前列腺素 F2α 的修饰
影响因子：
3.6
作者：
J. Nielsen;L. Fossom;S. Sparber
通讯作者：
S. Sparber
儿茶酚胺及相关化合物的高效液相色谱分离和检测。
DOI：
10.1016/0021-9673(75)85055-2
发表时间：
1979
期刊：
Journal of chromatography
影响因子：
--
作者：
G. A. Scratchley;A. N. Masoud;S. Stohs;D. Wingard
通讯作者：
D. Wingard
高效液相色谱法测定食物剥夺大鼠离散脑区的生物胺
影响因子：
3.6
作者：
C. Loullis;D. Felten;P. Shea
通讯作者：
P. Shea