Immunohistochemical localization of Toll-like receptors 2 and 4 in gingival tissue from patients with periodontitis
Immunohistochemical localization of Toll-like receptors 2 and 4 in gingival tissue from patients with periodontitis
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DOI：
10.1034/j.1399-302x.2003.180109.x
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发表时间：
2003-02-01
影响因子：
--
通讯作者：
Hara, Y
中科院分区：
文献类型：
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作者：
Mori, Y;Yoshimura, A;Hara, Y
本研究采用免疫组织化学方法研究了toll样受体（TLR） 2、TLR4、分化簇（cd14）和CD1a在人牙周炎牙龈中的表达。根据炎症程度将标本分为轻度、中度和重度三组。我们建立了三个区域，评估每个切片结缔组织中TLR2-、TLR4-、CD14-和cd1a阳性细胞占总细胞的比例。TLR2和TLR4在人牙周组织中表达，重度组1区（袋上皮下结缔组织）TLR2阳性细胞比例总体最高，重度组TLR4阳性细胞比例高于其他组。这些结果表明，TLR2和TLR4参与了牙周组织对细菌产物刺激的先天免疫反应。重症组1区cd14阳性细胞比例总体最低，CD1a阳性细胞比例高于其他各组。这些结果表明，CD14可能在炎症的发展过程中下调，并且/或者树突状细胞可能浸润慢性炎症的牙龈组织。
The present study investigated the expression of Toll-like receptor (TLR) 2, TLR4, cluster of differentiation (CD) 14 and CD1a in human periodontitis gingiva using immunohistochemical methods. The specimens were classified according to the degree of inflammation into three groups (mild, moderate and severe). We established three zones in which to evaluate the ratios of TLR2-, TLR4-, CD14- and CD1a-positive cells to total cells in the connective tissues of each section. TLR2 and TLR4 were expressed in human periodontal tissues, and the ratio of TLR2-positive cells was highest overall in zone 1 (connective tissue subjacent to pocket epithelium) of the severe group and that of TLR4-positive cells was higher in the severe group than in the other groups. These results suggest that TLR2 and TLR4 participate in the innate immune response to stimulation by bacterial products in periodontal tissues. The ratio of CD14-positive cells was lowest overall in zone 1 of the severe group and that of CD1a was higher in the severe group than in the other groups. These results suggest that CD14 may be down-regulated during the development of inflammation and/or dendritic cells might infiltrate chronically inflamed gingival tissue.