RPS2, AN ARABIDOPSIS DISEASE RESISTANCE LOCUS SPECIFYING RECOGNITION OF PSEUDOMONAS-SYRINGAE STRAINS EXPRESSING THE AVIRULENCE GENE AVRRPT2
RPS2, AN ARABIDOPSIS DISEASE RESISTANCE LOCUS SPECIFYING RECOGNITION OF PSEUDOMONAS-SYRINGAE STRAINS EXPRESSING THE AVIRULENCE GENE AVRRPT2
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DOI：
10.1105/tpc.5.8.865
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发表时间：
1993-08-01
期刊：
影响因子：
11.6
通讯作者：
STASKAWICZ, BJ
中科院分区：
文献类型：
--
作者：
KUNKEL, BN;BENT, AF;STASKAWICZ, BJ
利用分子遗传学方法对拟南芥中控制细菌性病害抗性的基因进行了鉴定和表征。筛选对细菌病原体假单胞菌致病变种具有改变抗性的突变体。表达无毒基因avrRpt 2的番茄（Pst）导致分离出四个敏感的rps（对P. dingae的抗性）突变体。rps突变体丧失了对表达avrRpt 2的细菌菌株的特异性抗性，因为它们保留了对表达无毒基因avrB或avrRpm 1的Pst菌株的抗性。遗传分析表明，在四个rps突变体的每一个，易感性是由于一个单一的突变映射到同一个位点上的4号染色体。鉴定出对单一细菌无毒基因具有特异性的抗性基因座表明，该基因座（命名为RPS 2）控制表达无毒基因avrRpt 2的细菌的特异性识别。生态型Wu-0是一种对表达avrRpt 2的Pst菌株易感的天然品系，似乎在RPS 2处缺乏功能等位基因，这表明在拟南芥野生种群中RPS 2基因座处存在天然变异。
A molecular genetic approach was used to identity and characterize plant genes that control bacterial disease resistance in Arabidopsis. A screen for mutants with altered resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) expressing the avirulence gene avrRpt2 resulted in the isolation of four susceptible rps (resistance to P. syringae) mutants. The rps mutants lost resistance specifically to bacterial strains expressing avrRpt2 as they retained resistance to Pst strains expressing the avirulence genes avrB or avrRpm1. Genetic analysis indicated that in each of the four rps mutants, susceptibility was due to a single mutation mapping to the same locus on chromosome 4. Identification of a resistance locus with specificity for a single bacterial avirulence gene suggests that this locus, designated RPS2, controls specific recognition of bacteria expressing the avirulence gene avrRpt2. Ecotype Wu-0, a naturally occurring line that is susceptible to Pst strains expressing avrRpt2, appears to lack a functional allele at RPS2, demonstrating that there is natural variation at the RPS2 locus among wild populations of Arabidopsis.