GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants
GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants
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DOI：
10.1016/j.phytochem.2006.07.021
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发表时间：
2006-10-01
期刊：
影响因子：
3.8
通讯作者：
Pare, Paul W.
中科院分区：
文献类型：
--
作者：
Farag, Mohamed A.;Ryu, Choong-Min;Pare, Paul W.
对芽孢杆菌菌株GB 03和IN 937 a的化学和植物生长研究表明，挥发性组分2，3-丁二醇和乙偶姻在拟南芥中触发植物生长促进。当细胞分裂素信号突变体暴露于GB 03与IN 937 a挥发物时，生长促进的差异表明这两种细菌菌株的化学信号的分歧。为了提供一个全面的化学档案的细菌挥发物从这些生物活性菌株，顶空固相微萃取（SPME）加上软件提取重叠的GC分离的成分。从GB 03和IN 937 a中鉴定出10种已报告的挥发性代谢物以及28种先前未表征的化合物。大多数新鉴定的化合物是从IN 937 a中释放的支链醇，其水平远高于GB 03。主成分分析清楚地将GB 03与IN 937 a分离，其中GB 03产生更高量的3-甲基-1-丁醇、2-甲基-1-丁醇和丁烷-1-甲氧基-3-甲基。支链醇与2，3-丁二醇具有相似的功能基序，并可能为细菌来源的激发子提供替代的结构模式。(c)2006爱思唯尔有限公司保留所有权利。
Chemical and plant growth studies of Bacilli strains GB03 and IN937a revealed that the volatile components 2,3-butanediol and acetoin trigger plant growth promotion in Arabidopsis. Differences in growth promotion when cytokinin-signaling mutants are exposed to GB03 versus IN937a volatiles suggest a divergence in chemical signaling for these two bacterial strains. To provide a comprehensive chemical profile of bacterial volatiles emitted from these biologically active strains, headspace solid phase microextraction (SPME) coupled with software extraction of overlapping GC-separated components was employed. Ten volatile metabolites already reported from GB03 and IN937a were identified as well as 28 compounds not previously characterized. Most of the newly identified compounds were branched-chain alcohols released from IN937a, at much higher levels than in GB03. Principal component analysis clearly separated GB03 from IN937a, with GB03 producing higher amounts of 3-methyl-1-butanol, 2-methyl-1-butanol and butane-1-methoxy-3-methyl. The branched-chain alcohols share a similar functional motif to that of 2,3-butanediol and may afford alternative structural patterns for elicitors from bacterial sources. (c) 2006 Elsevier Ltd. All rights reserved.