CD40 and IgE: synergism between anti-CD40 monoclonal antibody and interleukin 4 in the induction of IgE synthesis by highly purified human B cells.
CD40 and IgE: synergism between anti-CD40 monoclonal antibody and interleukin 4 in the induction of IgE synthesis by highly purified human B cells.
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CD40和IGE：抗CD40单克隆抗体和白介素4之间的协同作用在高度纯化的人B细胞诱导IgE合成中。
DOI：
10.1084/jem.172.6.1861
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发表时间：
1990-12-01
影响因子：
15.3
通讯作者：
Vercelli, D
中科院分区：
文献类型：
--
作者：
Jabara, H H;Fu, S M;Geha, R S;Vercelli, D
发现了一种新的IgE-B细胞分化途径。在重组白细胞介素4 （il -4）存在的情况下，单克隆抗体（mAb） 626.1的F（ab’）2片段与B细胞抗原CD40结合，可诱导高度纯化的人B细胞强烈合成IgE，但合成少量IgG。通过细胞分选分离的表面IgE- B细胞通过mAb 626.1和IL-4诱导产生IgE。因此，体内预置IgE的B细胞群的扩增不太可能导致IgE合成。一种中和性抗il -6抗体强烈抑制IgE反应，但不是完全抑制。这表明IL-6的自分泌在抗cd40单抗和IL- 4触发的IgE合成中起着重要的扩增作用。尽管CD40在体内的IgE反应中所起的确切作用仍有待确定，但这种T细胞无关的系统代表了一种有用的模型，可以表征人B细胞中导致IgE合成的生化和分子事件。
A novel pathway of IgE-B cell differentiation has been identified. Engagement of the B cell antigen CD40 by F(ab')2 fragments of monoclonal antibody (mAb) 626.1 in the presence of recombinant interleukin 4 (rIL-4) induced intense IgE synthesis, but modest IgG synthesis, by highly purified human B cells. Surface IgE- B cells isolated by cell sorting were induced to produce IgE by mAb 626.1 and IL-4. Thus, IgE synthesis is unlikely to result from expansion of a B cell population precommitted to IgE in vivo. A neutralizing anti-IL-6 antibody strongly, but not completely, inhibited the IgE response. This indicates that autocrine production of IL-6 plays an important amplification role in IgE synthesis triggered by anti-CD40 mAb and IL- 4. Although the exact role played by CD40 in IgE responses in vivo remains to be established, this T cell-independent system represents a useful model to characterize the biochemical and molecular events leading to IgE synthesis in human B cells.