Factors affecting the urease activity of native ureolytic bacteria isolated from coastal areas
Factors affecting the urease activity of native ureolytic bacteria isolated from coastal areas
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DOI：
10.12989/gae.2019.17.5.421
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发表时间：
2019-04-10
影响因子：
3.2
通讯作者：
Kawasaki, Satoru
中科院分区：
文献类型：
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作者：
Al Imran, Md;Nakashima, Kazunori;Kawasaki, Satoru
海岸侵蚀正在成为希腊、孟加拉国和全球的一个重大问题。为了防止和最大限度地减少海岸侵蚀造成的损害，传统上使用各种结构的组合。然而，这些方法中的大多数都是昂贵的。因此，使用当地的尿素分解细菌和微生物诱导碳酸盐沉淀（Microbially Induced Carbonate Precipitation，MICP）方法制造人工海滩岩石可以作为海岸侵蚀保护的替代方案，因为它是一种可持续和生态友好的生物地基改良技术。大多数关于MICP的研究都局限于陆地上的尿素分解细菌，而对沿海的尿素分解细菌用于测定尿素酶活性的研究却很少。因此，为了成功地将MICP应用于海岸侵蚀防护，细菌的类型、细菌细胞浓度、反应温度、细胞培养时间、碳酸盐沉淀趋势、控制脲酶活性的培养基的pH等，进行评估。在这项研究中，温度，pH值和培养时间的影响，以及在碳酸盐沉淀的趋势，沿海尿素分解细菌分离自两个沿海地区在希腊和孟加拉国，进行了评估。结果表明，沿海溶脲细菌的脲酶活性依赖于一些环境参数，这些参数对固沙的成功非常重要。在未来，我们的目标是将这些研究结果应用于创建人工海滩岩石结合土工织物管，用于地中海国家，孟加拉国和全球的海岸侵蚀保护，用于生物介导的土壤改良。
Coastal erosion is becoming a significant problem in Greece, Bangladesh, and globally. For the prevention and minimization of damage from coastal erosion, combinations of various structures have been used conventionally. However, most of these methods are expensive. Therefore, creating artificial beachrock using local ureolytic bacteria and the MICP (Microbially Induced Carbonate Precipitation) method can be an alternative for coastal erosion protection, as it is a sustainable and eco-friendly biological ground improvement technique. Most research on MICP has been confined to land ureolytic bacteria and limited attention has been paid to coastal ureolytic bacteria for the measurement of urease activity. Subsequently, their various environmental effects have not been investigated Therefore, for the successful application of MICP to coastal erosion protection, the type of bacteria, bacterial cell concentration, reaction temperature, cell culture duration, carbonate precipitation trend, pH of the media that controls the activity of the urease enzyme, etc., are evaluated. In this study, the effects of temperature, pH, and culture duration, as well as the trend in carbonate precipitation of coastal ureolytic bacteria isolated from two coastal regions in Greece and Bangladesh, were evaluated. The results showed that urease activity of coastal ureolytic bacteria species relies on some environmental parameters that are very important for successful sand solidification. In future, we aim to apply these findings towards the creation of artificial beachrock in combination with a geotextile tube for coastal erosion protection in Mediterranean countries, Bangladesh, and globally, for bio-mediated soil improvement.