Lipid bilayers at the gel interface for single ion channel recordings
Lipid bilayers at the gel interface for single ion channel recordings
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DOI：
10.1021/ac801224a
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发表时间：
2008-10-15
影响因子：
7.4
通讯作者：
Hirano, Minako
中科院分区：
文献类型：
--
作者：
Ide, Toru;Kobayashi, Toshihide;Hirano, Minako
使用人工脂质双分子层的单通道记录技术与膜片钳技术一样，是从生理学和药理学角度研究离子通道的一种非常强大的工具。它在研究那些用膜片吸管在技术上难以接近的通道时特别有优势。然而，双分子层的脆弱性以及将离子通道整合到其中的困难大大降低了测量效率。我们开发了一种在水凝胶表面形成人工脂质双分子层的新方法，该方法显著提高了测量效率。双分子层几乎瞬间（<1秒）形成，并能够在短时间（<30秒）内整合各种类型的离子通道蛋白，从而实现多通道测量。这些结果表明，这种方法有可能应用于开发用于药物设计的高通量筛选设备。
Single-channel recording using artificial lipid bilayers is along with the patch-clamp technique a very powerful tool to physiologically and pharmacologically study ion channels. It is particularly advantageous in studying channels that are technically difficult to access with a patch pipet. However, the fragility of the bilayers and the difficulty to incorporate ion channels into them significantly compromises measurement efficiency. We have developed a novel method for forming artificial lipid bilayers on a hydrogel surface that significantly improves the measurement efficiency. Bilayers formed almost instantly (< 1 s) and were able to incorporate various types of ion channel proteins within a short time (< 30 s) enabling multichannel measurements. These results indicate that this method can potentially be applied to developing high-throughput screening devices for drug design.