AUTOMATED ENZYMATIC ASSAYS FOR THE DETERMINATION OF INTESTINAL PERMEABILITY PROBES IN URINE .1. LACTULOSE AND LACTOSE
AUTOMATED ENZYMATIC ASSAYS FOR THE DETERMINATION OF INTESTINAL PERMEABILITY PROBES IN URINE .1. LACTULOSE AND LACTOSE
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DOI：
10.1016/0009-8981(90)90333-n
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发表时间：
1990-02-28
影响因子：
5
通讯作者：
BEHRENS, RH
中科院分区：
文献类型：
--
作者：
NORTHROP, CA;LUNN, PG;BEHRENS, RH
乳果糖正在成为评估小肠粘膜被动通透性的双糖的首选。然而，目前的分析方法既繁琐又耗时，阻碍了它更广泛的应用。本文介绍了一种自动分光光度测定尿液中乳果糖的技术，它通过一系列酶反应将乳果糖连接到NADPH的等摩尔产物上。除了乳果糖，该程序还提供了尿样中乳糖、葡萄糖和果糖的精确值。该测定已被证明对乳果糖和乳糖具有高度的特异性，不受高浓度其他糖或其他尿液成分的影响。批内和批间精密度与两种糖的变异系数在0.4-1.6%范围内相似。与现有的分析方法相比，该技术在时间、简单性和精确度方面都有了显著的改进。
Lactulose is becoming the disaccharide of choice in the dual sugar assessment of passive permeability of the small intestinal mucosa. However its more widespread use is hampered by current analytical methods which are tedious and time consuming. An automated spectrophotometric technique for the assay of this sugar in urine is presented in which lactulose is linked by a series of enzyme reactions to the equimolar production of NADPH. In addition to lactulose, the procedure also gives accurate values for lactose, glucose and fructose in the urine sample. The assay has been shown to be highly specific for lactulose and lactose and was not affected by high concentrations of other sugars or other urinary constituents. Within assay and between assay precision were similar with the coefficient of variation for both sugars in the range 0.4-1.6%. The technique represents a significant improvement in time, simplicity and precision on existing methods of analysis.