Methylated DNA collected by tampons--a new tool to detect endometrial cancer.

Methylated DNA collected by tampons--a new tool to detect endometrial cancer.
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DOI：
10.1158/1055-9965.882.13.5
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发表时间：
2004-05
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
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通讯作者：
H. Fiegl;Conny Gattringer;A. Widschwendter;Alois Schneitter;A. Ramoni;D. Sarlay;I. Gaugg;G. Goebel;H. Müller;E. Mueller-Holzner;C. Marth;M. Widschwendter
H. Fiegl;Conny Gattringer;A. Widschwendter;Alois Schneitter;A. Ramoni;D. Sarlay;I. Gaugg;G. Goebel;H. Müller;E. Mueller-Holzner;C. Marth;M. Widschwendter
中科院分区：
其他
文献类型：
--
作者：
H. Fiegl;Conny Gattringer;A. Widschwendter;Alois Schneitter;A. Ramoni;D. Sarlay;I. Gaugg;G. Goebel;H. Müller;E. Mueller-Holzner;C. Marth;M. Widschwendter

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这项原理性研究旨在确定一种新的、简单的使用表观遗传标记检测子宫内膜癌的策略。我们使用MethyLight研究了15例子宫内膜癌患者和109例非子宫内膜癌患者从子宫内膜收集的阴道分泌物中分离的DNA中5个基因（CDH13，HSPA2，MLH1，RASSF1A和SOCS2）的异常甲基化。所有子宫内膜癌患者均显示有三个或三个以上甲基化基因，而91%（109例中的99例）的非子宫内膜癌患者的阴道分泌物中没有或少于三个甲基化基因。本研究中开发的方法为前瞻性临床试验提供了基础，以筛选无症状的子宫内膜癌高危妇女。
This proof of principle study aimed to define a new and simple strategy for detection of endometrial cancer using epigenetic markers. We investigated DNA isolated from vaginal secretion collected from tampon for aberrant methylation of five genes (CDH13, HSPA2, MLH1, RASSF1A, and SOCS2) using MethyLight in 15 patients with endometrial cancer and 109 patients without endometrial cancer. All endometrial cancer patients revealed three or more methylated genes, whereas 91% (99 of 109) of the patients without endometrial cancer had no or fewer than three genes methylated in their vaginal secretion. The methods developed in this study provide the basis for a prospective clinical trial to screen asymptomatic women who are at high risk for endometrial cancer.