Microwave dielectric spectroscopy of cell membrane permeabilization with saponin on human B lymphoma cells
Microwave dielectric spectroscopy of cell membrane permeabilization with saponin on human B lymphoma cells
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微波介电谱法用皂苷对人 B 淋巴瘤细胞进行细胞膜透化
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
K. Grenier
中科院分区：
文献类型：
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作者：
F. Artis;D. Dubuc;J. Fournié;M. Poupot;K. Grenier
本文旨在为微波介电谱的生物应用相关性的示范。我们证明，微波介电谱确实可以显着识别细胞膜透化（及其对细胞的后果）的化学治疗诱导的细胞。真实的和虚部的标准偏差（分别）几个实验之间的介电常数分别低于0.08和0.06，而这两个参数的对比度分别达到1.92和1.30。10 GHz。与标准偏差比的对比度高于22，这使得生物学家相信该技术可以显著且有效地检测细胞群在其传统培养基中的活力状态。此外，这些结果允许预测，这种技术可以评估细胞群体的生存力，几乎没有准确性。
This paper aims to contribute to the demonstration of the microwave dielectric spectroscopy relevance for biological applications. We demonstrate indeed that microwave dielectric spectroscopy may significantly identify the membrane permeabilization (and its consequence on cells) of cells induced by chemo-treatment. Standard deviations on both real and imaginary parts (resp.) of the permittivity between several experiments are below 0.08 and 0.06 (resp.), whereas the contrasts on these two parameters reach 1.92 and 1.30 (resp.) at 10 GHz. The contrasts to the standard deviation ratio are then above 22, which permits to convince the biologists that the technique can significantly and efficiently detect the viability state of a cells population in their traditional culture medium. Moreover, these results permits to predict that such a technique can evaluate the viability of a cells population with few percents of accuracy.