Combined effects of sugarcane bagasse extract and synthetic dyes on the growth and bioaccumulation properties of Pichia fermentans MTCC 189.
Combined effects of sugarcane bagasse extract and synthetic dyes on the growth and bioaccumulation properties of Pichia fermentans MTCC 189.
复制标题
DOI：
10.1016/j.jhazmat.2010.07.051
复制
发表时间：
2010-11
影响因子：
13.6
通讯作者：
Devlina Das;D. Charumathi;N. Das
中科院分区：
文献类型：
--
作者：
Devlina Das;D. Charumathi;N. Das
研究了发酵毕赤酵母（Pichia fermentans MTCC 189）在甘蔗渣提取物培养基中对酸性蓝93、直接红28和碱性紫3的生物富集作用。最大的染料生物累积确定在pH 5.0的所有染料测试。为了确定染料对发酵假单胞菌MTCC 189的比生长速率的毒性作用，测试了两种动力学模型，即非竞争性和非竞争性模型。碱性紫3被发现比其他两种染料毒性更大。研究了甘蔗渣提取物和碱性紫3染料初始浓度对发酵假单胞菌MTCC 189的比生长速率和染料生物富集效率的联合影响，并采用响应面法（RSM）进行了优化。结果分析采用22完全析因中心复合设计。通过响应面法（RSM）预测的最佳组合表明，在含10 mg/L染料和24 g/L甘蔗渣糖的培养基中，发酵假单胞菌MTCC 189对碱性紫3染料的富集率可达69.8%。
Bioaccumulation of synthetic dyes viz. Acid Blue 93, Direct Red 28 and Basic Violet 3 by growing cells of yeast, Pichia fermentans MTCC 189 was investigated in growth media prepared from sugarcane bagasse extract. The maximum dye bioaccumulation was determined at pH 5.0 for all the dyes tested. Two kinetic models viz. Noncompetitive and Uncompetitive models were tested in order to determine the toxic effects of dyes on the specific growth rate of P. fermentans MTCC 189. Basic Violet 3 was found to be more toxic than the other two dyes. The combined effects of sugarcane bagasse extract and initial Basic Violet 3 dye concentrations on the specific growth rate and dye bioaccumulation efficiency of P. fermentans MTCC 189 was investigated and optimized using Response Surface Methodology (RSM). A 22full factorial central composite design was successfully used for analysis of results. The optimum combination predicted via RSM confirmed that P. fermentans MTCC 189 was capable of bioaccumulating Basic Violet 3 dye upto 69.8% in the medium containing 10mg/L of dye and 24g/L sugar extracted from sugarcane bagasse.