The Effect of Protozoa on the Composition of Rumen Bacteria in Cattle Using 16S rRNA Gene Clone Libraries
The Effect of Protozoa on the Composition of Rumen Bacteria in Cattle Using 16S rRNA Gene Clone Libraries
复制标题
DOI：
10.1271/bbb.69.499
复制
发表时间：
2005-01
期刊：
影响因子：
--
通讯作者：
Yuhei Ozutsumi;K. Tajima;A. Takenaka;H. Itabashi
中科院分区：
文献类型：
--
作者：
Yuhei Ozutsumi;K. Tajima;A. Takenaka;H. Itabashi
研究了原生动物的存在对牛瘤胃细菌组成的影响。将7头阉割的荷斯坦牛分为4头阉割和3头未阉割两组，构建16S核糖体RNA基因克隆文库。共对312个克隆进行了1500 bp的测序。将151个序列划分为98个操作分类单元（otu），相似性至少为97%。其中厚壁菌门（59.7%）、拟杆菌门（34.4%）、螺旋体菌门（2.6%）、放线菌门（2.0%）和变形菌门（1.3%）。拟杆菌和普氏菌是放牧牛的主要菌群，占34.4%。161个序列被划分为72个otu。从非同源文库中得到的序列分为厚壁菌门（65.7%）、拟杆菌门（31.1%）、变形菌门（1.9%）和螺旋体（1.2%）。肉毒梭菌群及其近缘菌群（36.0%）是无头牛的主要菌群。计算机程序LIBSHUFF的分析表明，瘤胃原虫的存在显著影响了瘤胃细菌的组成。
The effect of the presence of protozoa on the composition of rumen bacteria was investigated in cattle. Seven castrated Holstein cattle were divided into two groups: four faunated and three unfaunated, and 16S ribosomal RNA gene (rDNA) clonal libraries were constructed. A total of 312 clones were sequenced across 1,500 bp. The 151 sequences of the faunated cattle were classified into 98 operational taxonomic units (OTUs) having at least 97% similarity. The sequences derived from the faunated cattle were classified into Firmicutes (59.7%), Bacteroidetes (34.4%), Spirochaetes (2.6%), Actinobacteria (2.0%), and Proteobacteria (1.3%). Bacteroides and Prevotella (34.4%) were the major groups in the faunated cattle. The 161 sequences in the unfaunated cattle were classified into 72 OTUs. The sequences derived from the unfaunated libraries were classified into Firmicutes (65.7%), Bacteroidetes (31.1%), Proteobacteria (1.9%), and Spirochaetes (1.2%). The Clostridium botulinum group and its relatives (36.0%) were the major groups in the unfaunated cattle. An analysis by the computer program LIBSHUFF clarified that the presence of ruminal protozoa markedly affected the composition of rumen bacteria.