The 10-nm chromatin fiber and its relationship to interphase chromosome organization.
The 10-nm chromatin fiber and its relationship to interphase chromosome organization.
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DOI：
10.1042/bst20170101
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发表时间：
2018-02-19
影响因子：
3.9
通讯作者：
Maeshima K
中科院分区：
文献类型：
--
作者：
Hansen JC;Connolly M;McDonald CJ;Pan A;Pryamkova A;Ray K;Seidel E;Tamura S;Rogge R;Maeshima K
染色体是由核小体和蛋白质沿长度方向组装的单一的长DNA分子。在间期，哺乳动物的染色体以高度组织的超分子球体形式存在于细胞核中。在这里，我们讨论关于基因组DNA是如何在间期染色体内包装和组织的新见解。我们的重点是染色体组织的结构原理，特别关注10 nm染色质纤维的内在贡献，而不是常规的30 nm纤维。我们假设，间期染色体的层次化球状组织从根本上是由10 nm的核小体之字形阵列的自我相互作用特性建立的，而组蛋白翻译后修饰、组蛋白变体和染色质相关蛋白则用于将通用的染色质结构域塑造成特定的结构和功能实体。
A chromosome is a single long DNA molecule assembled along its length with nucleosomes and proteins. During interphase, a mammalian chromosome exists as a highly organized supramolecular globule in the nucleus. Here, we discuss new insights into how genomic DNA is packaged and organized within interphase chromosomes. Our emphasis is on the structural principles that underlie chromosome organization, with a particular focus on the intrinsic contributions of the 10-nm chromatin fiber, but not the regular 30-nm fiber. We hypothesize that the hierarchical globular organization of an interphase chromosome is fundamentally established by the self-interacting properties of a 10-nm zig-zag array of nucleosomes, while histone post-translational modifications, histone variants, and chromatin-associated proteins serve to mold generic chromatin domains into specific structural and functional entities.