Heat shock protein 90 co-chaperone modules fine-tune the antagonistic interaction between salicylic acid and auxin biosynthesis in cassava.
Heat shock protein 90 co-chaperone modules fine-tune the antagonistic interaction between salicylic acid and auxin biosynthesis in cassava.
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DOI：
10.1016/j.celrep.2021.108717
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发表时间：
2021-02
期刊：
影响因子：
8.8
通讯作者：
Yunxie Wei;Binbin Zhu;Wen Liu;Xiao Cheng;Daozhe Lin;Chaozu He;Haitao Shi
中科院分区：
文献类型：
--
作者：
Yunxie Wei;Binbin Zhu;Wen Liu;Xiao Cheng;Daozhe Lin;Chaozu He;Haitao Shi
热休克蛋白90（HSP 90）是植物中重要的分子伴侣。然而，在木薯中，HSP 90介导的植物免疫应答仍然是难以捉摸的。在本研究中，木薯细菌性枯萎病（CBB）诱导MeHsf 8的表达，MeHsf 8直接靶向MeHSP 90.9以激活其表达和免疫应答。进一步鉴定SHI相关序列1（MeSRS 1）和MeWRKY 20作为MeHSP 90.9共伴侣，揭示了MeHSP 90.9介导的免疫应答的潜在机制。MeHSP90.9与MeSRS 1和MeWRKY 20相互作用，促进其分别转录激活水杨酸（SA）生物合成基因PphB易感基因3（MePBS 3）和色氨酸代谢基因N-乙酰羟色胺O-甲基转移酶2（MeASMT 2），从而激活SA生物合成，抑制拟南芥生长素生物合成。值得注意的是，遗传实验证实，过表达MePBS 3和MeASMT 2可以挽救沉默MeHsf 8-MeHSP90.9对抗病性的影响。本研究强调了MeHSP 90.9对SA和生长素生物合成的双重调节，提供了对MeHSP 90.9客户伙伴在植物免疫中的机制的理解。
Heat shock protein 90 (HSP90) is an important molecular chaperone in plants. However, HSP90-mediated plant immune response remains elusive in cassava. In this study, cassava bacterial blight (CBB) induces the expression ofMeHsf8, which directly targetsMeHSP90.9to activate its expression and immune response. Further identification of SHI-related sequence 1 (MeSRS1) and MeWRKY20 as MeHSP90.9 co-chaperones revealed the underlying mechanism of MeHSP90.9-mediated immune response. MeHSP90.9 interacts with MeSRS1 and MeWRKY20 to promote their transcriptional activation of salicylic acid (SA) biosynthetic geneavrPphB Susceptible 3(MePBS3) and tryptophan metabolic geneN-acetylserotonin O-methyltransferase 2(MeASMT2), respectively, so as to activate SA biosynthesis but inhibit tryptophan-derived auxin biosynthesis. Notably, genetic experiments confirmed that overexpressingMePBS3andMeASMT2could rescue the effects of silencing MeHsf8-MeHSP90.9 on disease resistance. This study highlights the dual regulation of SA and auxin biosynthesis by MeHSP90.9, providing the mechanistic understanding of MeHSP90.9 client partners in plant immunity.