Further definition of the Ly-5 system.
Further definition of the Ly-5 system.
复制标题
Ly-5系统的进一步定义。
DOI：
10.1007/bf00364741
复制
发表时间：
1986
期刊：
影响因子：
3.2
通讯作者：
Boyse,EA
中科院分区：
文献类型：
--
作者：
Shen,FW;Tung,JS;Boyse,EA
Ly-5由发育的造血分支细胞表达。在Ly-5单克隆抗体和两个Ly-5同源小鼠品系的辅助下，进一步对Ly-5系统进行血清学分析，发现了两个新的Ly-5同种抗原，Ly-5。3和Ly-5.4。结果表明，胸腺细胞有三种表型，即Ly-5.1，3、Ly-5.2，4和Ly-5.2，3，相应的基因型分别为Ly-5a、Ly-5 b和Ly-5c，其中Ly-5a是最常见的等位基因。TheLy-5愈伤组织仅在ST/bJ株中发现，这一发现与以前用Ly-5 cDNA探针对ST/bJ DNA进行Southern印迹时观察到的目前独特的限制性片段模式一致。目前的血清学和生物化学数据有利于解释，胸腺细胞的复合赖氨酸-5表型是由于两个单独的赖氨酸-5分子亚型，表现出离散的蛋白质组成的差异，承担不同的赖氨酸-5抗原，并共同产生的胸腺细胞，不像其他赖氨酸-5亚型先前显示区分不同的造血细胞谱系。
Ly-5is expressed by cells of the hematopoietic branch of development. Further serological analysis of theLy-5system, aided by Ly-5 monoclonal antibodies and by twoLy-5congenic mouse strains, reveals two new Ly-5 alloantigens, Ly-5. 3 and Ly-5.4. The data define three thymocyte phenotypes, Ly-5.1,3, Ly-5.2,4, and Ly-5.2,3, and three corresponding genotypes,Ly-5a,Ly-5b, andLy-5c, respectively.Ly-5ais by far the most common allele. TheLy-5callele is found only in the ST/bJ strain, a finding that accords with the presently unique pattern of restriction fragments previously observed in Southern blotting of ST/bJ DNA with an Ly-5 cDNA probe. Present serological and biochemical data favor the interpretation that the compound Ly-5 phenotype of thymocytes is attributable to two separate Ly-5 molecular isoforms that exhibit a discrete difference in protein composition, bear different Ly-5 antigens, and are produced jointly by thymocytes, unlike other Ly-5 isoforms previously shown to distinguish different hematopoietic cell lineages.