Genetic Divergence between Freshwater and Marine Morphs of Alewife (Alosa pseudoharengus): A 'Next-Generation' Sequencing Analysis
Genetic Divergence between Freshwater and Marine Morphs of Alewife (Alosa pseudoharengus): A 'Next-Generation' Sequencing Analysis
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DOI：
10.1371/journal.pone.0031803
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发表时间：
2012-03-15
期刊：
影响因子：
3.7
通讯作者：
Michalak, Pawel
中科院分区：
文献类型：
--
作者：
Czesny, Sergiusz;Epifanio, John;Michalak, Pawel
一种原产于大西洋的小型团头鱼，最近(与150年前类似)入侵了北美五大湖，尽管面临淡水环境的挑战，但其数量激增，扰乱了当地的食物网络结构。范围的扩大伴随着各级组织的戏剧性变化。生长速度、成熟时的大小或繁殖力只是对比这两种妻子的形态和生活史特征的几个最明显的特征。一个问题是，海洋和淡水品种之间的这些快速演变的差异在多大程度上是由调节性(包括表型可塑性)或结构突变造成的。为了深入了解海洋和淡水鸟类之间的表达变化和序列差异，我们对密歇根湖和大西洋的个体的转录本进行了测序。群体特有的单核苷酸多态很少见，但有趣的是，发生在基因序列中的基因也往往表现出很大的表达差异。我们的结果表明，淡水型和湖型之间的显著表型差异可以归因于大量的调控修改，而不是编码的变化。
Alewife Alosa pseudoharengus, a small clupeid fish native to Atlantic Ocean, has recently (similar to 150 years ago) invaded the North American Great Lakes and despite challenges of freshwater environment its populations exploded and disrupted local food web structures. This range expansion has been accompanied by dramatic changes at all levels of organization. Growth rates, size at maturation, or fecundity are only a few of the most distinct morphological and life history traits that contrast the two alewife morphs. A question arises to what extent these rapidly evolving differences between marine and freshwater varieties result from regulatory (including phenotypic plasticity) or structural mutations. To gain insights into expression changes and sequence divergence between marine and freshwater alewives, we sequenced transcriptomes of individuals from Lake Michigan and Atlantic Ocean. Population specific single nucleotide polymorphisms were rare but interestingly occurred in sequences of genes that also tended to show large differences in expression. Our results show that the striking phenotypic divergence between anadromous and lake alewives can be attributed to massive regulatory modifications rather than coding changes.