BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients.
BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients.
复制标题
BMP9 诱导来自颅缝早闭患者的永生化人颅缝祖细胞的成骨和脂肪生成
DOI：
10.1111/jcmm.13193
复制
发表时间：
2017-11
影响因子：
5.3
通讯作者：
He TC
中科院分区：
文献类型：
--
作者：
Song D;Zhang F;Reid RR;Ye J;Wei Q;Liao J;Zou Y;Fan J;Ma C;Hu X;Qu X;Chen L;Li L;Yu Y;Yu X;Zhang Z;Zhao C;Zeng Z;Zhang R;Yan S;Wu T;Wu X;Shu Y;Lei J;Li Y;Zhang W;Wang J;Lee MJ;Wolf JM;Huang D;He TC
颅缝复合体是由成骨前体细胞和骨髓间充质干细胞(MSCs)和缝合间充质组成的异质组织。颅缝融合在发育过程中是一个高度协调和严格调控的过程。颅缝融合是一种因颅缝过早融合而导致的先天性畸形。虽然来源于颅缝复合体的祖细胞对于研究骨缝发育和致病性早期缝合融合的分子机制很有价值，但原代人颅缝祖细胞的寿命有限，并在传代过程中逐渐丧失成骨能力。为了克服为骨缝生物学研究维持充足和长期培养的骨缝祖细胞的技术挑战，我们建立并鉴定了可逆性永生化的人颅缝祖细胞(ISuPs)。使用带有FRT位点的表达SV40T的可逆永生化系统，我们证明了来自颅突融合患者专利缝合的原代人类缝合祖细胞可以有效地永生化。在体外和体内BMP9刺激下，iSuPs保持长期增殖活性，表达大多数MSC标志物，并可分化为成骨和成脂细胞系。FLP重组酶去除SV40T抗原后，细胞增殖下降，内源性成骨和成脂能力增强。因此，iSups是研究颅缝发育、膜内成骨和颅缝融合的发病机制以及探索颅骨组织工程的有价值的资源。
The cranial suture complex is a heterogeneous tissue consisting of osteogenic progenitor cells and mesenchymal stem cells (MSCs) from bone marrow and suture mesenchyme. The fusion of cranial sutures is a highly coordinated and tightly regulated process during development. Craniosynostosis is a congenital malformation caused by premature fusion of cranial sutures. While the progenitor cells derived from the cranial suture complex should prove valuable for studying the molecular mechanisms underlying suture development and pathogenic premature suture fusion, primary human cranial suture progenitors (SuPs) have limited life span and gradually lose osteoblastic ability over passages. To overcome technical challenges in maintaining sufficient and long‐term culture of SuPs for suture biology studies, we establish and characterize the reversibly immortalized human cranial suture progenitors (iSuPs). Using a reversible immortalization system expressing SV40 T flanked with FRT sites, we demonstrate that primary human suture progenitor cells derived from the patent sutures of craniosynostosis patients can be efficiently immortalized. The iSuPs maintain long‐term proliferative activity, express most of the consensus MSC markers and can differentiate into osteogenic and adipogenic lineages upon BMP9 stimulation in vitro and in vivo. The removal of SV40 T antigen by FLP recombinase results in a decrease in cell proliferation and an increase in the endogenous osteogenic and adipogenic capability in the iSuPs. Therefore, the iSuPs should be a valuable resource to study suture development, intramembranous ossification and the pathogenesis of craniosynostosis, as well as to explore cranial bone tissue engineering.
登录
查看更多内容
条件永生化小鼠胚胎成纤维细胞保留增殖活性而不影响多能分化潜能
影响因子：
3.7
作者：
Huang E;Bi Y;Jiang W;Luo X;Yang K;Gao JL;Gao Y;Luo Q;Shi Q;Kim SH;Liu X;Li M;Hu N;Liu H;Cui J;Zhang W;Li R;Chen X;Shen J;Kong Y;Zhang J;Wang J;Luo J;He BC;Wang H;Reid RR;Luu HH;Haydon RC;Yang L;He TC
通讯作者：
He TC
生长激素通过激活小鼠多系细胞中的 JAK/STAT/IGF1 通路与 BMP9 协同促进成骨分化
影响因子：
6.2
作者：
Huang, Enyi;Zhu, Gaohui;He, Tong-Chuan
通讯作者：
He, Tong-Chuan
影响因子：
5.1
作者：
Kang, Q;Sun, MH;He, TC
通讯作者：
He, TC
胰岛素样生长因子 2 (IGF-2) 增强 BMP-9 诱导的成骨分化和骨形成
影响因子：
6.2
作者：
Chen, Liang;Jiang, Wei;Huang, Jiayi;He, Bai-Cheng;Zuo, Guo-Wei;Zhang, Wenli;Luo, Qing;Shi, Qiong;Zhang, Bing-Qiang;Wagner, Eric R.;Luo, Jinyong;Tang, Min;Wietholt, Christian;Luo, Xiaoji;Bi, Yang;Su, Yuxi;Liu, Bo;Kim, Stephanie H.;He, Connie J.;Hu, Yawen;Shen, Jikun;Rastegar, Farbod;Huang, Enyi;Gao, Yanhong;Gao, Jian-Li;Zhou, Jian-Zhong;Reid, Russell R.;Luu, Hue H.;Haydon, Rex C.;He, Tong-Chuan;Deng, Zhong-Liang
通讯作者：
Deng, Zhong-Liang
影响因子：
3.5
作者：
Desai, J;Shannon, ME;Culiat, CT
通讯作者：
Culiat, CT