High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study
High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study
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DOI：
10.1038/sj.bjc.6604503
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发表时间：
2008-07-29
影响因子：
8.8
通讯作者：
Al Moustafa, A-E
中科院分区：
文献类型：
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作者：
Akil, N.;Yasmeen, A.;Al Moustafa, A-E
高危型人乳头瘤病毒（HPV）可能是乳腺癌发生和转移的重要危险因素。基于这一假设，我们最近研究了高危型HPV 16的E6/E7癌蛋白在两种非侵袭性人乳腺癌细胞系BT 20和MCF 7中的作用;我们报告了E6/E7将这些细胞系转化为侵袭性细胞。这伴随着Id-1的过表达，Id-1是乳腺转移的重要调节因子。在这项调查中，我们研究了高危HPV（16，18，31，33和35）的存在和E6癌蛋白的表达，以及它们与Id-1基因表达的相关性，分别使用聚合酶链反应（PCR）和组织芯片（TMA）分析，在一个队列的113例叙利亚乳腺癌患者。我们发现高危型HPV 16、18、31、33和35分别存在于我们样本的8.84%、9.73%、7.07%、55.75%和37.16%中，代表浸润性乳腺癌。总的来说，69（61.06%）的113例标本中的HPV阳性，其中24例（34.78%）的组织中同时感染一种以上的HPV类型。此外，我们报告说，这些高危HPV的E6癌蛋白的表达与Id-1过表达在大多数浸润性乳腺癌组织样本。我们的数据表明，高风险HPV感染与叙利亚妇女的人类乳腺癌进展有关。
High-risk human papillomaviruses (HPVs) could be important risk factors for breast carcinogenesis and metastasis. Based on this hypothesis, we recently studied the effect of E6/E7 onco-proteins of high-risk HPV type 16 in two non-invasive human breast cancer cell lines, BT20 and MCF7; we reported that E6/E7 converts these cell lines to invasive cells. This is accompanied by an overexpression of Id-1, which is an important regulator of breast metastasis. In this investigation, we examined the presence of high-risk HPVs ( 16, 18, 31, 33 and 35) and the expression of their E6 onco-protein as well as their correlation with Id-1 gene expression, using polymerase chain reaction (PCR) and tissue microarray (TMA) analysis, respectively, in a cohort of 113 Syrian breast cancer patients. We found that high-risk HPV types 16, 18, 31, 33 and 35 are present in 8.84, 9.73, 7.07, 55.75 and 37.16% of our samples, respectively, which represent invasive breast cancers. Overall, 69 (61.06%) of the 113 samples are HPV positive; among these specimens 24 tissues (34.78%) are coinfected with more than one HPV type. Furthermore, we report that the expression of the E6 onco-protein of these high-risk HPVs is correlated with Id-1 overexpression in the majority of invasive breast cancer tissue samples. Our data suggest that high-risk HPV infections are associated with human breast cancer progression in Syrian women.