Genomic structure of the human bradykinin B1 receptor gene and preliminary characterization of its regulatory regions.
Genomic structure of the human bradykinin B1 receptor gene and preliminary characterization of its regulatory regions.
复制标题
人缓激肽 B1 受体基因的基因组结构及其调控区域的初步表征。
DOI：
10.1006/bbrc.1996.0810
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Polgar,P
中科院分区：
文献类型：
--
作者：
Yang,X;Polgar,P
我们报道了从人肺成纤维细胞基因组文库中分离的人BK B1受体基因。该基因以单拷贝形式存在，跨越10多个碱基，包括三个外显子，被两个内含子中断。使用RNase保护试验和基因组DNA和cDNA序列匹配绘制外显子的相对位置。对外显子、内含子-外显子边界及5′、3′侧翼区进行测序。5′端非翻译区分布在所有三个外显子上，而编码区完全位于第三个外显子上。外显子-内含子连接序列高度保守。引物延伸分析映射的转录起始位点21碱基对下游的TATA序列。在人BK B1受体基因中发现了两个Alucs序列和两个功能不同的启动子。
We report on the isolation of the human BK B1receptor gene from a human lung fibroblast genomic library. The gene is present as a single copy, spans more than 10 kilobases and includes three exons interrupted by two introns. The relative position of the exons was mapped using RNase protection assay, and genomic DNA and cDNA sequence matches. Exon and intron-exon boundaries, and 5′, 3′ flanking regions were sequenced. While the 5′ untranslated region is distributed on all three exons, the coding region is located entirely on the third exon. The exon-intron junction sequences are highly conserved. Primer extension analysis mapped the transcriptional initiation site 21 base pairs downstream of a TATA sequence. Two Alucsequences and two distinct functional promoters were found in the human BK B1receptor gene.