Exercise training improves liver steatosis in mice.
Exercise training improves liver steatosis in mice.
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DOI：
10.1186/s12986-015-0026-1
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发表时间：
2015
影响因子：
4.5
通讯作者：
Kersten S
中科院分区：
文献类型：
--
作者：
Alex S;Boss A;Heerschap A;Kersten S
非酒精性脂肪性肝病（NAFLD）正迅速成为世界上最常见的肝脏疾病。已被证明有效改善NAFLD的策略之一是定期运动，这似乎在一定程度上独立于减肥来降低脂肪变性。然而，关于所涉及的机制的数据有限。本研究的目的是确定规律运动对肝脏脂肪变性影响的机制。非肥胖的雄性小鼠通过喂食富含蔗糖的缺乏胆碱的饮食而变得脂肪变性。然后，它们每天在跑步机上跑步三周，而对照组小鼠则保持静止不动。与未训练的小鼠相比，训练后的小鼠脂肪组织质量相似，但肝脏脂滴大小显著减少，肝脏甘油三酯含量降低（~ 30%,P < 0.05）。各种血脂参数和血糖水平在训练小鼠和未训练小鼠之间相似，而肝糖原水平在训练小鼠中明显更高。两组小鼠的肝脏甘油三酯分泌率和新生脂肪生成没有变化，脂肪分解和自噬指标也没有变化。最后，全基因组表达谱显示，运动训练对ppar α介导的氧化代谢（包括脂肪酸氧化）调节有轻微的刺激作用。综上所述，我们的研究表明，反复运动降低肝脏脂肪变性可能是由于肝脏中燃料氧化途径的激活。
Non-alcoholic fatty liver disease (NAFLD) is rapidly turning into the most common liver disorder worldwide. One of the strategies that has been shown to effectively improve NAFLD is regular exercise, which seems to lower steatosis partly independent of weight loss. However, limited data are available about the mechanisms involved. The aim of the present study was to identify the mechanisms underlying the effect of regular exercise on liver steatosis. Non-obese male mice were rendered steatotic by feeding a sucrose-enriched choline-deficient diet. They were then subjected to daily treadmill running for three weeks, whereas the control mice remained sedentary. Compared to the untrained mice, trained mice showed similar adipose tissue mass but had significantly reduced size of lipid droplets in the liver coupled with a reduction in liver triglyceride content (~30 %, P < 0.05). Levels of various plasma lipid parameters and plasma glucose were similar between the trained and untrained mice, whereas levels of hepatic glycogen were significantly higher in the trained mice. Hepatic triglyceride secretion rate and de novo lipogenesis were unchanged between the two sets of mice, as were indicators of lipolysis and autophagy. Finally, whole genome expression profiling indicated a mild stimulatory effect of exercise training on PPARα-mediated regulation of oxidative metabolism, including fatty acid oxidation. Taken together, our study suggests that the lowering of hepatic steatosis by repeated exercise is likely due to activation of fuel oxidation pathways in liver.