Levels of thioredoxin are related to the severity of obstructive sleep apnea: based on oxidative stress concept
Levels of thioredoxin are related to the severity of obstructive sleep apnea: based on oxidative stress concept
复制标题
硫氧还蛋白水平与阻塞性睡眠呼吸暂停的严重程度相关：基于氧化应激概念
DOI：
10.1007/s11325-012-0692-4
复制
发表时间：
2013-03-01
影响因子：
2.5
通讯作者：
Wan, Huan Ying
中科院分区：
文献类型：
--
作者：
Guo, Qian;Wang, Yan;Wan, Huan Ying
研究背景氧化应激是阻塞性睡眠呼吸暂停（obstructive sleep apnea，OSA）的典型特征。硫氧还蛋白（TRX）作为氧化应激的生物标志物之一，是一种有效的抗氧化防御蛋白质二硫键还原酶。我们的研究旨在测试是否硫氧还蛋白可以评估严重程度的OSA. MethodsSixthy成人怀疑有OSA被列入本研究，并根据多导睡眠图（PSG）的结果分为四组：对照组，轻度，中度，重度。排除患有慢性内科疾病（原发性高血压除外）或服用任何抗氧化药物的受试者。在过夜PSG测试后1小时内采集血样。结果重症组血浆TRX水平明显升高（8.62 ± 2.14，13.33 ± 5.60，14.71 ± 5.53，16.10 ± 7.34ng/ml;p< 0.05）; TRX与AHI呈正相关（r= 0.313;p< 0.05），与最低血氧饱和度呈负相关（r= 0.266;p< 0.037）。伴有高血压的OSA患者血清TRX水平明显升高（17.70 ± 6.98vs.13.43 ± 5.83ng/ml;t= 2.434，p < 0.018）。TRX诊断OSA的临界值为9.39 ng/ml（敏感性91%，特异性78%），鉴别中重度OSA和轻度OSA的临界值为11.79 ng/ml（敏感性75%，特异性65%）。因此，TRX可用作OSA的严重性指标。
BackgroundOxidative stress is a typical feature of obstructive sleep apnea (OSA). Thioredoxin (TRX), as one of the oxidative stress biomarkers, is a potent protein disulfide reductase in antioxidant defense. Our study is designed to test whether thioredoxin could assess the severity of OSA.MethodsSixty-three adults suspected of having OSA were included in this study and were divided into four groups based on the results of polysomnography (PSG): control, mild, moderate, and severe. Subjects with chronic medical diseases (with the exception of essential hypertension) or taking any antioxidant medication were excluded. Blood samples were obtained within an hour after the overnight PSG test. Plasma TRX levels were detected by enzyme-linked immunosorbent assay.ResultsThe plasma TRX level in severe group was obviously increased (8.62 ± 2.14, 13.33 ± 5.60, 14.71 ± 5.53, and 16.10 ± 7.34 ng/ml;p< 0.05). The TRX positively related to AHI (r= 0.313;p< 0.05), while negatively related to the lowest O2saturation (r= 0.266;p< 0.037). The OSA patients associated with hypertension showed elevated TRX level (17.70 ± 6.98 vs. 13.43 ± 5.83 ng/ml;t= 2.434,p< 0.018). The cutoff value of TRX for identifying OSA was 9.39 ng/ml (sensitivity 91 %, specificity 78 %), and its cutoff value for differentiating moderate–severe OSA from mild OSA was 11.79 ng/ml (sensitivity 75 %, specificity 65 %).ConclusionThese results suggest that plasma TRX level is associated with the severity of OSA. Therefore, TRX may be used as a severity indicator of OSA.