[Analysis of genetic difference among Scrophularia ningpoensis cultivars by SRAP].
[Analysis of genetic difference among Scrophularia ningpoensis cultivars by SRAP].
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发表时间：
2009
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通讯作者：
Da-Xia Chen;Long-yun Li;Rui Peng;Yekuan Wu;Yingfan Cai
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作者：
Da-Xia Chen;Long-yun Li;Rui Peng;Yekuan Wu;Yingfan Cai
目的从分子水平分析宁波宁玄参品种间的遗传差异。方法采用序列相关扩增多态性（SRAP）方法对3个宁波南栽培品种92个个体进行分析。利用POPGENE1.31计算参数，并基于UPGMA方法构建关系。结果1)共获得227条条带，其中多态性条带199条。2)结果表明，3个品种间的遗传多样性处于中等水平。在种水平上，多态性位点百分比PPB=52.42%，有效等位基因数N(e)=1.2812, Nei’s基因多样性H=0.1671, Shannon’s信息指数H(sp)= 0.2526；在品种水平上，PPB=21.44%， N(e)=1.1216, Nei’s基因多样性H=0.0725, Shannon’s信息指数H(pop)= 0.1083。3) Nei’s遗传分化系数为0.5625，与Shannon’s遗传分化系数（0.5713）一致。大部分遗传变异存在于品种间。4)品种间的基因流量（N(m)=0.3889）较小，说明品种间的遗传分化程度较高。5)遗传相似系数由0.8082变为0.9133。聚类分析表明，SRAP标记与传统模态性状的分类结果基本一致。结论南水北调的遗传多样性为中等水平。品种间遗传差异大于品种内遗传差异。
OBJECTIVE The genetic difference among Scrophularia ningpoensis cultivars were analyzed in molecular level. METHOD Ninety-two individuls of three S. ningpoensis cultivars were employed to be analyzed by the approach of Sequence-related Amplified Polymorphism (SRAP).The parameters were calculated by POPGENE1.31 and the relationship was constructed based on UPGMA method. RESULT 1) A total of 227 bands were scored and 199 bands of them were polymorphic. 2) The result is showed that there is a medium level of genetic diversity among three cultivars. At species level: percentage of polymorphic loci PPB=52.42%, effective number of alleles N(e)=1.2812, Nei's gene diversity H=0.1671 and Shannon's information index H(sp)= 0.2526; At cultivar level: PPB=21.44%, N(e)=1.1216, Nei's gene diversity H=0.0725 and Shannon's information index H(pop)= 0.1083. 3) The Nei's coefficient of genetic differentiation was 0.5625, which was consistent with the Shannon's coefficient of genetic differentiation (0.5713). Most of the genetic variation existed among cultivars. 4) The gene flow (N(m)=0.3889) was less among cultivars, indicating that the degree of genetic differentiation was higher. 5) Genetic similarity coefficient were changed from 0.8082 to 0.9133. By clustering analysis, the classified result of SRAP marker between traditional modal character was almost same. CONCLUSION The genetic diversity of samples of S. ningpoensis is medium. The genetic difference among cultivar is higher than that within cultivar.