Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences
Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences
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DOI：
10.1002/jbt.21467
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发表时间：
2013-01-01
影响因子：
3.6
通讯作者：
Poole, Jill A.
中科院分区：
文献类型：
--
作者：
Golden, Gregory A.;Wyatt, Todd A.;Poole, Jill A.
暴露于有机粉尘会诱发呼吸道炎症性疾病。维生素D最近已与各种气道炎症性疾病，但其在农业有机粉尘暴露中的作用尚不清楚。这项研究调查了维生素D是否减少细胞培养和动物模型中有机粉尘诱导的炎症结果。从猪隔离设施获得的有机粉尘提取物诱导中性粒细胞趋化因子的产生（人IL-8，鼠CXCL 1/CXCL 2）。用1，25-（OH）2D 3预处理的人血单核细胞、人支气管上皮细胞和鼠肺切片中的中性粒细胞趋化因子诱导减少。鼻内吸入有机粉尘提取物诱导中性粒细胞流入，与低维生素D饮食的小鼠相比，喂食相对高维生素D饮食的小鼠中CXCL 1/CXCL 2释放也减少。这些发现与气管上皮细胞PKCa和PKCe活性降低以及全肺TLR 2和TLR 4基因表达相关。总的来说，维生素D在调节有机粉尘诱导的气道炎症结果中起作用。(C)2012 Wiley Periodicals，Inc. J BiochemMol Toxicol 27：77-86，2013;在wileyonlinelibrary.com在线查看这篇文章。DOI 10.1002/jbt.21467
Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dustinduced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)2D3. Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCa and PKCe activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dustinduced airway inflammatory outcomes. (C) 2012 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:77-86, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21467