Comparative quantitative proteomics to investigate the remodeling of bioenergetic pathways under iron deficiency in Chlamydomonas reinhardtii
Comparative quantitative proteomics to investigate the remodeling of bioenergetic pathways under iron deficiency in Chlamydomonas reinhardtii
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DOI：
10.1002/pmic.200700407
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发表时间：
2007-11-01
期刊：
影响因子：
3.4
通讯作者：
Hippler, Michael
中科院分区：
文献类型：
--
作者：
Naumann, Bianca;Busch, Andreas;Hippler, Michael
在这项研究中解决的基本问题是如何调整能量代谢，以科普铁缺乏在莱茵衣藻。为了研究缺铁对生物能量途径的影响，将比较蛋白质组学与光谱和伏安氧测量相结合，以评估与呼吸和光合机制的功能特性相关的蛋白质动力学。虽然光合电子传递在很大程度上受到损害铁缺乏，我们的定量和光谱数据显示，光系统11（PSII）的功能天线的大小显着增加。与此同时，胁迫相关的叶绿体多肽，如2-cys peroxiredoxin和胁迫诱导的捕光蛋白，LhcSR 3，以及一种新的捕光蛋白和几种未知功能的蛋白质诱导铁剥夺。呼吸耗氧量并没有减少，因此，呼吸复合物的多肽，窝藏大量的铁硫簇，只有轻微减少，甚至增加低铁。因此，铁剥夺诱导的过渡，从photolieterotrophic主要是异养代谢，表明一个层次的铁分配在一个细胞的细胞器内存在的是密切相关的细胞的代谢状态。
The basic question addressed in this study is how energy metabolism is adjusted to cope with iron deficiency in Chlamydomonas reinhardtii. To investigate the impact of iron deficiency on bioenergetic pathways, comparative proteornics was combined with spectroscopic as well as voltametric oxygen measurements to assess protein dynamics linked to functional properties of respiratory and photosynthetic machineries. Although photosynthetic electron transfer is largely compromised under iron deficiency, our quantitative and spectroscopic data revealed that the functional antenna size of photosystem 11 (PSII) significantly increased. Concomitantly, stressrelated chloroplast polypeptides, like 2-cys peroxiredoxin and a stress-inducible light-harvesting protein, LhcSR3, as well as a novel light-harvesting protein and several proteins of unknown function were induced under iron-deprivation. Respiratory oxygen consumption did riot decrease and accordingly, polypeptides of respiratory complexes, harboring numerous iron-sulfur clusters, were only slightly diminished or even increased under low iron. Consequently, iron-deprivation induces a transition from photolieterotrophic to primarily heterotrophic metabolism, indicating that a hierarchy for iron allocations within organelles of a single cell exists that is closely linked with the metabolic state of the cell.