Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells
Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells
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DOI：
10.1016/s0014-5793(03)01505-9
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发表时间：
2004-01-30
期刊：
影响因子：
3.5
通讯作者：
Eccles, MR
中科院分区：
文献类型：
--
作者：
Muratovska, A;Eccles, MR
短干扰RNA（siRNA）的高效递送为研究细胞功能提供了强有力的方法。将siRNA偶联到膜渗透肽（MPPs）穿透素和转运素以提高它们被细胞的摄取。合成对应于荧光素酶或绿色荧光蛋白（GFP）转基因的含巯基siRNA，并通过在细胞质的还原环境中不稳定的二硫键与穿膜蛋白或转运蛋白偶联。这些MPP-siRNA在高比例的细胞中有效地减少了几种哺乳动物细胞类型中报告转基因的瞬时和稳定表达，并表现出与阳离子脂质体相当或更好的递送特性，操作更少。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.(C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.