Association between chemical and genetic variation of Vitex rotundifolia populations from different locations in China:: its implication for quality control of medicinal plants
Association between chemical and genetic variation of Vitex rotundifolia populations from different locations in China:: its implication for quality control of medicinal plants
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DOI：
10.1002/bmc.841
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发表时间：
2007-09-01
影响因子：
1.8
通讯作者：
Zheng, Hanchen
中科院分区：
文献类型：
--
作者：
Hu, Yuan;Zhang, Qiaoyan;Zheng, Hanchen
圆叶荆条是一种分布广泛的植物，在中药中有着广泛的应用。其果实牡荆子在《中国药典》中记载为芒井子。为了对化学变异模式所反映的药用价值进行有效的质量控制，以及与遗传多样性的关系，本研究对中国不同产地的14个圆叶杜鹃居群进行了基于高效液相指纹图谱和ISSR分子标记的分析。高效液相色谱数据显示，圆叶委陵菜居群间的化学成分存在较大差异。系统聚类分析进一步根据它们的化学型变异分为四大类群。根据ISSR数据，圆叶云杉居群具有丰富的遗传多样性，并被聚为4个类群。值得注意的是，分子标记揭示的遗传变异模式与圆叶葡萄果实的化学成分所指示的遗传变异模式密切相关。这一发现为结合使用化学指纹图谱和遗传指纹图谱有效地评价圆叶月桂的品质和选择具有适当药理特性的有利化学类型提供了坚实的基础，此外还为建立良好的药用植物农业实践提供了坚实的基础。版权所有(C)2007 John Wiley&Sons，Ltd.
Vitex rotundifolia is a widely distributed plant species that has been extensively used in traditional Chinese medicine. Its fruits, Fructus Viticis, are recorded as Manjingzi in the Pharmacopoeia of the People's Republic of China. For the effective quality control of its medicinal values reflected by chemical variation patterns, in addition to the relationship with genetic diversity, analyses based on high-performance liquid chromatographic (HPLC) fingerprinting and inter simple sequence repeat (ISSR) molecular markers were carried out, involving 14 V. rotundifolia populations from different locations in China. The HPLC data showed considerable variation of chemical constituents among the V. rotundifolia populations. The hierarchical clustering analysis further revealed four major groups based on their chemotype variation. Abundant genetic diversity was detected among the V. rotundifolia populations that also were clustered into four groups based on their ISSR data. It is important to point out that the genetic variation pattern revealed by molecular markers was closely associated with that indicated by chemical constitutes in the fruits of V. rotundifolia. This finding provides a solid basis for the combined use of chemical and genetic fingerprints in efficiently evaluating qualities and choosing favourable chemotypes with appropriate pharmacological properties of V. rotundifolia, in addition to establishing good agricultural practices for medicinal plants. Copyright (c) 2007 John Wiley & Sons, Ltd.