Effects of macrolides on antigen presentation and cytokine production by dendritic cells and T lymphocytes
Effects of macrolides on antigen presentation and cytokine production by dendritic cells and T lymphocytes
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DOI：
10.1016/j.ijporl.2006.10.016
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发表时间：
2007-02-01
影响因子：
1.5
通讯作者：
Harabuchi, Yasuaki
中科院分区：
文献类型：
--
作者：
Ishida, Yoshiya;Abe, Yusuke;Harabuchi, Yasuaki
大环内酯类药物是治疗慢性鼻窦炎、支气管窦综合征和弥漫性泛细支气管炎等慢性呼吸道疾病的有效药物。虽然对其机制的了解有限，但据报道，多种炎症细胞因子（IL-8等）和一些转录因子的抑制参与其中。不可分型流感嗜血杆菌（NTHI）是呼吸道最重要的病原体之一。P6是NTHI的外膜蛋白之一，是保护性抗体的靶抗原。为了分析大环内酯类药物对人树突状细胞（dc）的影响，我们用大环内酯类药物处理dc，并将其作为抗原呈递细胞（APCs）。克拉霉素、罗红霉素和强的松龙抑制CD4+ T细胞对P6的体外增殖反应和细胞因子的产生。作为对照，我们也单独培养DCs并将其暴露于药物中，而相反地培养T细胞而不向培养物中添加任何药物。结果显示了类似的抑制免疫反应的趋势。这些结果表明，大环内酯类药物在体外抑制树突状细胞抗原特异性免疫反应。2006爱思唯尔爱尔兰有限公司版权所有。
Macrolides are effective therapeutic agents for chronic respiratory tract diseases, such as chronic sinusitis, sinobronchial syndrome and diffuse panbronchiolitis. Although only limited information is available about their mechanisms, suppression of various inflammatory cytokines (IL-8, etc.) and some transcription factors has been reported to be involved. Non-typeable Haemophilus influenzae (NTHI) is one of the most important pathogens of the respiratory tract. P6 is one of the outer membrane proteins of NTHI and the target antigen of protective antibodies. To analyze the influence of macrolides on human dendritic cells (DCs), we treated DCs with macrolides and used them as antigen-presenting cells (APCs). Clarithromycin, roxithromycin and prednisolone suppressed the in vitro proliferative response of CD4+ T cells to P6 and also the production of cytokines. As a control, we also cultured DCs alone and exposed them to the medicament, while conversely culturing T cells without adding any drugs to the cultures. The results showed similar tendencies for suppression of immune responses. These findings suggest that macrolides suppress the antigen-specific immune responses of DCs in vitro. (C) 2006 Elsevier Ireland Ltd. All rights reserved.