Resveratrol modulates murine collagen-induced arthritis by inhibiting Th17 and B-cell function
Resveratrol modulates murine collagen-induced arthritis by inhibiting Th17 and B-cell function
复制标题
DOI：
10.1136/ard.2011.149831
复制
发表时间：
2012-01-01
影响因子：
27.4
通讯作者：
Xu, Damo
中科院分区：
文献类型：
--
作者：
Gao Xuzhu;Komai-Koma, Mousa;Xu, Damo
背景：酒精摄入量与类风湿关节炎（RA）的发病率和严重程度呈负相关。白藜芦醇是一种安全的、被充分描述的植物源化合物，具有抗炎和免疫调节特性，存在于红葡萄酒中。因此，它可以介导后者的抗炎特性，并提供新的治疗效用是自己right.Objective白藜芦醇对胶原诱导的关节炎（CIA）的治疗作用和其假定的免疫modulation in mice.Methods CIA诱导DBA 1小鼠与胶原II免疫。在CIA发生之前或之后给予不同剂量的白藜芦醇。抗体和细胞因子的水平在血清中或在引流淋巴结（DLN）淋巴细胞培养上清液中测定ELISA和DLN中的Th 17细胞的发展进行了监测流式cytometer.Results无论是预防性或治疗性管理白藜芦醇衰减CIA小鼠的临床参数和骨侵蚀。关节炎的保护作用与显着降低血清水平的促炎细胞因子和胶原蛋白特异性，但不是总的，IgG，并与减少数量的Th 17细胞和IL-17的生产DLN.Conclusion白藜芦醇调制炎症性关节炎啮齿动物通过选择性抑制关键的细胞和体液反应疾病的发展。这可以部分解释红葡萄酒的保护作用，但重要的是可以提供一种新的，有效的和安全的途径，从而可以开发新的药物来治疗RA。
Background Alcohol intake is inversely related to rheumatoid arthritis (RA) disease incidence and severity. Resveratrol, a safe, well-described plant-derived compound, possesses anti-inflammation and immune-regulatory properties and is present in red wine. As such, it could mediate anti-inflammatory properties of the latter and offer novel therapeutic utility in is own right.Objective To evaluate the therapeutic effect of resveratrol on collagen-induced arthritis (CIA) and its putative immune modulation in mice.Methods CIA was induced in DBA1 mice by immunisation with collagen II. Different doses of resveratrol were administered before or after the development of CIA. The levels of antibody and cytokines in serum or in draining lymph node (DLN) lymphocyte culture supernatants were measured by ELISA and Th17 cell development in DLN was monitored by flow cytometry.Results Either prophylactic or therapeutic administration of resveratrol attenuated clinical parameters and bone erosion in CIA mice. The arthritis-protective effects were associated with markedly reduced serum levels of pro-inflammatory cytokines and collagen-specific, but not total, IgG, and with reduced numbers of Th17 cells and the production of IL-17 in DLN.Conclusion Resveratrol modulates inflammatory arthritis in rodents by selectively suppressing key cellular and humoral responses necessary for disease development. This may partly explain the protective effects of red wine but importantly may offer a novel, effective and safe pathway whereby novel agents could be developed to treat RA.