Transcript levels of five enzymes involved in cortisol synthesis and regulation during the stress response in common carp: Relationship with cortisol
Transcript levels of five enzymes involved in cortisol synthesis and regulation during the stress response in common carp: Relationship with cortisol
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DOI：
10.1016/j.ygcen.2009.05.006
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发表时间：
2009-10-01
影响因子：
2.7
通讯作者：
Komen, J.
中科院分区：
文献类型：
--
作者：
Nematollahi, M. A.;van Pelt-Heerschap, H.;Komen, J.
本研究以鲤鱼（Cyprinus carpio L.）研究了对3小时净限制应力的响应，然后恢复22小时。测定血浆中皮质醇、葡萄糖、乳酸盐和游离脂肪酸水平。基于鲤科同源物鉴定星星、P450 c17 a2、3 β HSD、P450 c21和11 β HSD 2转录物序列，并通过实时PCR定量。结果表明，应激后1h血浆皮质醇水平达到峰值，是对照组的85倍，恢复后4 h迅速恢复正常。首次在肾间质中鉴定出11种β HSD 2转录本。在限制期间和限制后皮质醇水平的变化与11 β HSD 2 mRNA水平的增加和减少分别呈时间延迟关系。结果表明，皮质醇可能参与了11 β-HSD 2的调控或激活。StAR和P450 c21 mRNA水平在净限制应激和恢复过程中无明显变化，而P450 c17 a2在恢复后4和22 h显著升高。由于血浆皮质醇水平增加了68倍，在5分钟内净限制应力，似乎这种酶的转录激活并不直接参与急性皮质醇的生产。(C)2009爱思唯尔公司All rights reserved.
In this study the expression of five genes involved in cortisol synthesis and regulation in the head kidneys of common carp (Cyprinus carpio L.) has been investigated in response to 3 h net confinement stress, followed by 22 h recovery. Cortisol, glucose, lactate and free fatty acid levels were measured in blood plasma. StAR, P450c17a2, 3 beta HSD, P450c21 and 11 beta HSD2 transcript sequences were identified based on Cyprinidae homologs and quantified by real-time PCR. Results showed that the plasma cortisol level reached a peak at one hour post-stress (85-fold higher than in control) and quickly returned to normal after 4 h recovery. 11 beta HSD2 transcripts were for the first time identified in interrenals. Changes in cortisol levels during and after confinement were correlated in a time-delayed relationship with increase and decrease in mRNA levels of 11 beta HSD2, respectively. These results suggest that cortisol may be involved in the control or activation of 11 beta HSD2.StAR and P450c21 mRNA levels did not change during net confinement stress and recovery, but P450c17a2 levels were significantly increased 4 and 22 h after recovery. Since plasma cortisol levels increased by 68-fold within 5 min net confinement stress, it seems that transcriptional activation of this enzyme is not directly involved in acute cortisol production. (C) 2009 Elsevier Inc. All rights reserved.