Regulation of mouse pancreatitis-associated protein-I gene expression during caerulein-induced acute pancreatitis.
Regulation of mouse pancreatitis-associated protein-I gene expression during caerulein-induced acute pancreatitis.
复制标题
雨蛙素诱导的急性胰腺炎期间小鼠胰腺炎相关蛋白 I 基因表达的调节。
作者：
Fu,K;SarrasJr,MP;DeLisle,RC;Andrews,GK
胰腺炎相关蛋白（PAP）是急性胰腺炎期间主要的胰腺分泌蛋白。然而，人们对 PAP 基因表达的调控机制知之甚少，并且缺乏有关小鼠 PAP 基因表达的信息。在此，我们采用Northern印迹和RNase保护测定来测量正常胰腺和肠中以及雨蛙素诱导的急性胰腺炎期间的胰腺中的小鼠PAP-1 mRNA水平。出乎意料的是，我们发现小鼠 PAP-1 mRNA 水平在成年胰腺以及小肠中持续较高。此外，在开始注射雨蛙素后，小鼠胰腺PAP-1 mRNA水平迅速且显着下调(3小时)，但到72小时缓慢恢复到高水平。有趣的是，我们发现在施用L-丁硫氨酸-[S，R]-亚磺酰亚胺后，胰腺PAP-I mRNA水平也短暂且显着下调。因此，证明了小鼠胰腺中PAP-1 mRNA水平和谷胱甘肽水平之间的相关性。
The pancreatitis-associated proteins (PAPs) are major pancreatic secretory proteins during acute pancreatitis. However, mechanisms of regulation of PAP gene expression are poorly understood, and there is a lack of information regarding mouse PAP gene expression. Herein, we employed Northern blotting and RNase protection assays to measure mouse PAP-I mRNA levels in the normal pancreas and intestine, and in the pancreas during caerulein-induced acute pancreatitis. Unexpectedly, we found that mouse PAP-I mRNA levels are constitutively high in the adult pancreas, as well as in the small intestine. Furthermore, mouse pancreatic PAP-I mRNA levels are rapidly and dramatically down-regulated (3 h) after the initiation of caerulein injections, but slowly return to high levels by 72 h. Interestingly, we found that pancreatic PAP-I mRNA levels are also transiently and dramatically down-regulated afterL-buthionine-[S, R]-sulfoximine administration. Thus, a correlation between PAP-I mRNA levels and glutathione levels in the mouse pancreas was demonstrated.