Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins.
Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins.
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DOI：
10.1083/jcb.200105044
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发表时间：
2001-11-26
期刊：
影响因子：
--
通讯作者：
Jockusch BM
中科院分区：
文献类型：
--
作者：
Hüttelmaier S;Illenberger S;Grosheva I;Rüdiger M;Singer RH;Jockusch BM
以黏着斑蛋白/后黏着斑蛋白的羧基端片段为诱饵，从酵母双杂交文库中筛选到一个新蛋白raver 1。Raver 1是一个80 kD的多结构域蛋白，在不同的细胞系中广泛表达，但表达量不同。在原位和体外，raver 1与微丝相关蛋白纽蛋白、元纽蛋白和α-辅肌动蛋白形成复合物，并与纽蛋白/元纽蛋白和α-辅肌动蛋白共定位在微丝附着位点，例如上皮细胞和成纤维细胞的细胞-细胞和细胞基质接触，分别在骨骼肌的肋肌中。raver 1的NH 2-末端部分包含三个RNA识别基序，与异质核RNP（hnRNP）家族的成员具有同源性。Raver 1与多聚嘧啶束结合蛋白（PTB）/hnRNPI（一种参与微丝蛋白RNA剪接的蛋白质）共定位于核仁周围区室，并与PTB/hnRNPI形成复合物。因此，raver 1是一个双室蛋白，这与其序列中核定位信号和核输出序列基序的存在是一致的。在肌肉分化过程中，raver 1从细胞核迁移到肋节。我们建议，raver 1可以协调RNA加工和靶向所需的微丝锚定在特定的粘附位点。
By screening a yeast two-hybrid library with COOH-terminal fragments of vinculin/metavinculin as the bait, we identified a new protein termed raver1. Raver1 is an 80-kD multidomain protein and widely expressed but to varying amounts in different cell lines. In situ and in vitro, raver1 forms complexes with the microfilament-associated proteins vinculin, metavinculin, and α-actinin and colocalizes with vinculin/metavinculin and α-actinin at microfilament attachment sites, such as cell–cell and cell matrix contacts of epithelial cells and fibroblasts, respectively, and in costameres of skeletal muscle. The NH2-terminal part of raver1 contains three RNA recognition motifs with homology to members of the heterogeneous nuclear RNP (hnRNP) family. Raver1 colocalizes with polypyrimidine tract binding protein (PTB)/hnRNPI, a protein involved in RNA splicing of microfilament proteins, in the perinucleolar compartment and forms complexes with PTB/hnRNPI. Hence, raver1 is a dual compartment protein, which is consistent with the presence of nuclear location signal and nuclear export sequence motifs in its sequence. During muscle differentiation, raver1 migrates from the nucleus to the costamere. We propose that raver1 may coordinate RNA processing and targeting as required for microfilament anchoring in specific adhesion sites.
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影响因子：
14.9
作者：
GHETTI, A;PINOLROMA, S;DREYFUSS, G
通讯作者：
DREYFUSS, G
影响因子：
64.5
作者：
Bakolitsa, C;de Pereda, JM;Liddington, RC
通讯作者：
Liddington, RC
DOI：
10.1073/pnas.95.26.15293
发表时间：
1998-12-22
影响因子：
11.1
作者：
Fan, XHC;Steitz, JA
通讯作者：
Steitz, JA
DOI：
10.1083/jcb.118.6.1411
发表时间：
1992-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Danowski BA;Imanaka-Yoshida K;Sanger JM;Sanger JW
通讯作者：
Sanger JW
影响因子：
11.4
作者：
Conte, MR;Grüne, T;Curry, S
通讯作者：
Curry, S