Expression alteration of long non-coding RNAs and their target genes in the intestinal mucosa of patients with Crohn’s disease
Expression alteration of long non-coding RNAs and their target genes in the intestinal mucosa of patients with Crohn’s disease
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DOI：
10.1016/j.cca.2019.02.031
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
施瑞华
中科院分区：
文献类型：
--
作者：
李娜;施瑞华
Backgroud: CD(Crohn's Disease)is a chronic idiopathic inflammatory disorders of the GI tract. It is increasing.worldwide and has become a global health problem. The key pathological mechanism for CD occurrence has not.been identified, and present treatments are mostly anti-symptom therapy, which has limited efficacy. In this.study, we investigate whether lncRNAs are involved in the pathogenesis of CD and how they may regulate the.target genes in CD process..Methods: CD patients were diagnosed in Zhongda Hospital of Southeast University between May 2017 and May.2018. Pathological and normal intestinal mucosa were collected and total RNA was extracted the expression of.lncRNA and mRNA was profiled and analyzed by using lncRNA and mRNA gene chips. The lncRNAs and mRNAs.with significant alternations (≥10 times and P < 0.05) were identified and verified. The co-expressed mRNAs.with the differentially expressed lncRNAs were revealed by CNC analysis. The potential regulatory factors were.determined by the Ce (cis/tans) mechanism analysis with the use of miRbase, Targetscan, and NCBI database..Finally, the lncRNA-miRNA/TF-mRNA expression network was predicted..Results: Eight lncRNAs were found to be differentially expressed between the pathological mucosa and the.normal mucosas in the ileal end. CNC analysis of the differentially expressed lncRNAs revealed fifty co-expressed.mRNAs with positive or negative regulation. Base on the mRNAs KEGG pathway analysis, most of them appeared.to be involved in cell signaling pathways. Six lncRNAs in the cytoplasm participated in the Ce mechanism, and.the rest two lncRNAs in the nucleus participated in the cis/trans regulation mechanism. Finally, ternary relationship of lncRNAs-miRNAs and TFs-mRNAs was obtained by CNC,KEGG enrichment analysis and Ce (trans/.cis) analysis..Conclusion: The differential expression of lncRNAs in CD mucosa indicated that lncRNAs were involved in immune reaction. These lncRNAs might contribute to the regulation of intestinal mucosa function through the.genetic network of lncRNAs -miRNAs/TFs-mRNAs.
