Investigation into the metabolism of 1,8‐cineole in an intestinal cell culture model and acquisition of its immune‐modulatory effect via gene expression analysis
Investigation into the metabolism of 1,8‐cineole in an intestinal cell culture model and acquisition of its immune‐modulatory effect via gene expression analysis
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研究肠细胞培养模型中 1,8-桉叶素的代谢并通过基因表达分析获得其免疫调节作用
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发表时间：
2012
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通讯作者：
M. Pfaffl
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作者：
J. Müller;N. Gruner;Isabella Almstätter;Frauke Kirsch;A. Buettner;M. Pfaffl
1，8-桉叶素是一种常用的具有消炎和抗炎作用的香料，本研究主要针对其肠道作用对其潜在的生理作用进行了研究。因此，这项研究的目的是利用组合方法学方法在化学分析的基础上监测潜在的生物转化效应，以及利用生理学和免疫学工具监测生物反馈的进一步影响。用逆转录实时定量聚合酶链式反应监测1，8-桉叶油素治疗后特定标记基因的相对表达变化。此外，用阻抗传感技术研究了1，8-桉叶素对肠道细胞增殖和适合性的潜在影响。一般来说，我们的研究表明，应用的模型系统没有导致任何显著的代谢物形成，也没有应用剂量导致任何关于基因表达的重大改变。此外，在药理或生理浓度下，桉叶油素对猪肠道上皮细胞没有影响；在附着和铺展过程中也没有影响，对融合细胞层也没有影响。只有接触高浓度桉叶素(>；1 g/L)才会影响细胞并导致大量细胞脱落。总体而言，我们的研究表明，即使是常见的较高剂量的1，8-桉叶素似乎也不会导致任何主要的生理或厌恶反应，直到达到一个临界浓度，然后直接导致肠道模型中的细胞死亡。版权所有©2012 John Wiley&Sons，Ltd.
1,8-Cineole, a common and widely used odorant with antiphlogistic and anti-inflammatory properties, was investigated in this study with regard to potential physiological effects targeting mainly its intestinal effects. Accordingly, the aim of the study was to utilize a combinatory methodological approach to both monitor potential biotransformatory effects on a chemo-analytical basis, as well as physiological and immunological tools to monitor further effects of biofeedback. Reverse transcription quantitative real-time polymerase chain reaction was used to monitor the occurrence of relative expression changes for particular marker genes, following 1,8-cineole treatment. Furthermore, a potential effect of 1,8-cineole on the proliferation and fitness of the intestinal cells using impedance sensing was studied. Generally, our studies showed that the applied model system did neither lead to any significant metabolite formation, nor did the applied dosages result in any major modifications with regard to gene expression. Also, it was shown that cineole had no effect on the intestinal porcine epithelial cells applied in pharmacological or physiological concentrations; neither during the attachment and spreading process nor on confluent cell layers. Only the exposure to high concentrations of cineole (> 1 g/l) affected the cells and led to massive cell detachment. Overall, our studies show that even common higher 1,8-cineole dosages do not seem to lead to any major physiological or aversive response, only until a critical concentration is reached that then directly leads to cell death within the intestinal model. Copyright © 2012 John Wiley & Sons, Ltd.