Progesterone and modulation of endothelium-dependent responses in canine coronary arteries.
Progesterone and modulation of endothelium-dependent responses in canine coronary arteries.
复制标题
黄体酮和犬冠状动脉内皮依赖性反应的调节。
DOI：
10.1152/ajpregu.1991.261.4.r1022
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
--
作者：
Miller,VM;Vanhoutte,PM
雌激素长期治疗可增强某些内皮依赖性舒张作用。孕酮是否会产生类似的效果尚不清楚。设计实验以确定孕酮长期治疗对内皮依赖性反应的影响。切除成年雌性犬的卵巢，皮下植入含有载体物质、雌激素、孕激素或雌激素加孕激素的丸剂。14-21天后，取出冠状动脉，切成环，并在吲哚美辛存在下悬挂以测量器官腔室中的等长力。ADP、缓激肽或钙离子载体的内皮依赖性舒张在各组之间没有差异。然而，雌激素治疗组对乙酰胆碱和α 2-肾上腺素能激动剂BHT-920的舒张作用大于雌激素+炔雌醇治疗组。在没有内皮的环中，对一氧化氮和异丙肾上腺素的舒张作用在各组之间没有差异。然而，舒张的平滑肌ADP是更大的孕酮治疗组比孕酮+雌激素组。这些结果表明，孕酮单独影响内皮依赖性反应最小。然而，孕酮似乎拮抗雌激素对两种内皮依赖性反应的刺激作用，这两种反应与百日咳毒素敏感的鸟嘌呤核苷酸调节蛋白和一氧化氮的产生有关。这些研究表明，一个特定的受体/第二信使系统可以由女性生殖类固醇激素调节。
Chronic treatment with estrogens enhances some endothelium-dependent relaxations. Whether or not progesterone would exert a similar effect is unknown. Experiments were designed to determine the effect of chronic treatment with progesterone on endothelium-dependent responses. Adult female dogs were ovariectomized and pellets containing carrier substance, estrogen, progesterone, or estrogen plus progesterone were implanted subcutaneously. After 14-21 days coronary arteries were removed, cut into rings, and suspended for the measurement of isometric force in organ chambers in the presence of indomethacin. Endothelium-dependent relaxations to ADP, bradykinin, or the calcium ionophore did not differ among groups. However, relaxations to acetylcholine and to the alpha 2-adrenergic agonist BHT-920 were greater in the estrogen-treated group than in the estrogen plus progesterone-treated group. In rings without endothelium, relaxations to nitric oxide and isoproterenol did not differ among groups. However, relaxations of the smooth muscle to ADP were greater in the progesterone-treated group than in the progesterone plus estrogen group. These results suggest that progesterone alone minimally affects endothelium-dependent responses. However, progesterone seems to antagonize the stimulatory effects of estrogen on two endothelium-dependent responses that are associated with pertussis toxin-sensitive guanine nucleotide regulatory proteins and the production of nitric oxide. These studies suggest that a specific receptor/second messenger system can be modulated by female reproductive steroid hormones.