REORGANIZATION OF SUBSTRATUM-BOUND FIBRONECTIN ON HYDROPHILIC AND HYDROPHOBIC MATERIALS IS RELATED TO BIOCOMPATIBILITY
REORGANIZATION OF SUBSTRATUM-BOUND FIBRONECTIN ON HYDROPHILIC AND HYDROPHOBIC MATERIALS IS RELATED TO BIOCOMPATIBILITY
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DOI：
10.1007/bf00120366
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发表时间：
1994-09-01
影响因子：
3.7
通讯作者：
GROTH, T
中科院分区：
文献类型：
--
作者：
ALTANKOV, G;GROTH, T
一般的趋势是，哺乳动物细胞与生物表面的相互作用比与非生物表面的相互作用更好。这种差异的基础仍然知之甚少。在这项研究中，亲水性清洁玻璃和疏水性十八烷基玻璃已被用作模型表面。我们发现，亲水性表面上的成纤维细胞可以重组荧光纤连蛋白（FN）的细胞外基质样结构，而在疏水性表面上没有重排的FN发生。这伴随着成纤维细胞在清洁玻璃上的高度增殖，而在十八烷基玻璃上没有细胞生长。此外，它表明，有显着的差异，粘附到亲水性和疏水性表面的成纤维细胞的形态，判断的整体细胞形状，FN受体和肌动蛋白丝的组织。事实上，FN在这些表面上的预吸附几乎可以消除亲水性和疏水性表面之间的形态差异。然而，FN的预吸附不能恢复成纤维细胞在疏水表面上的增殖。综上所述，结果表明FN的吸附和重组方法可能对材料的生物相容性至关重要。
It is a general trend that mammalian cells interact better with wettable surfaces than with non-wettable surfaces. The basis for this difference is still poorly understood. In this study hydrophilic clean glass and hydrophobic octadecyl glass have been used as model surfaces. We show that fibroblasts on hydrophilic surfaces may reorganize fluorescent fibronectin (FN) in an extracellular matrix-like structure whereas on hydrophobic surfaces no rearrangement of FN occurs. This was accompanied by a high proliferation of fibroblasts on clean glass whereas on octadecyl glass no cell growth occurred. Moreover, it was demonstrated that there are striking differences in the morphology of fibroblasts adhering to hydrophilic and hydrophobic surfaces, judged by the overall cell shape, the organization of FN receptors and actin filaments. Indeed, the preadsorption of FN on these surfaces could almost abolish morphological differences between hydrophilic and hydrophobic surfaces. However, preadsorption of FN could not restore the proliferation of fibroblasts on the hydrophobic surface. Taken together, the results suggest that the method of adsorption and reorganization of FN may be critical for the biocompatibility of materials.