Differentiation of smooth muscle phenotypes in mouse mesangial cells.
Differentiation of smooth muscle phenotypes in mouse mesangial cells.
复制标题
小鼠系膜细胞平滑肌表型的分化。
作者：
N. Iehara;H. Takeoka;H. Tsuji;T. Imabayashi;D. Foster;A. Strauch;Y. Yamada;T. Kita;T. Doi
平滑肌α-肌动蛋白（SMA）mRNA，血管平滑肌细胞的标志物，在体内和体外正常肾小球系膜被确定。研究了几个系膜细胞群体，以确定SMA和基底膜胶原是否一起调节。SMA的表达水平，这可能与分化阶段，是不同的细胞群体的不同。一个细胞群体在其早期传代时具有高SMA和IV型胶原蛋白水平。其他的表达间质和基底膜胶原。第一个群体在后来的传代中发展出这些表型特征。SMA和α 1（IV）胶原蛋白表达水平共同调节，而α 2（I）胶原蛋白水平与SMA和α 1（IV）胶原蛋白表达相反。SMA和IV型胶原均由顺式调节剂的甲基化状态控制;然而，I型胶原主要由反式调节剂调节。用5-氮杂胞苷处理将成纤维细胞表型的细胞转化为平滑肌细胞样表型。这些细胞系可用于研究体外分化过程。
Smooth muscle alpha-actin (SMA) mRNA, a marker of vascular smooth muscle cells, was identified in the normal glomerular mesangium both in vivo and in vitro. Several populations of mesangial cells were studied to determine if SMA and basement membrane collagen were regulated together. The levels of SMA expression, which could be linked to the stage of differentiation, were different for the differing cell populations. One cell population had high SMA and type IV collagen levels at its early passages. The others expressed both interstitial and basement membrane collagens. The first population developed these phenotypic features at later passages. The levels of SMA and alpha 1(IV) collagen expression were regulated together in concert, whereas the alpha 2(I) collagen levels were expressed inversely to SMA and alpha 1(IV) collagen. Both SMA and type IV collagen were controlled by the methylation states of the cis-regulators; however, type I collagen was mainly regulated by the trans-acting regulators. Treatment with 5-azacytidine converted the cells of a fibroblast-phenotype to a smooth muscle cell-like phenotype. These cell lines may be useful for studying the differentiation process in vitro.
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DOI：
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发表时间：
1992-04
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
J. Floege;C. Alpers;M. Burns;P. Pritzl;K. Gordon;W. Couser;Richard J. Johnson
通讯作者：
J. Floege;C. Alpers;M. Burns;P. Pritzl;K. Gordon;W. Couser;Richard J. Johnson
体内大鼠主动脉平滑肌细胞异肌动蛋白表达的发育变化。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Owens,GK;Thompson,MM
通讯作者：
Thompson,MM
顺式平滑肌转录激活所需的平滑肌 α-肌动蛋白启动子元件。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Blank,RS;McQuinn,TC;Yin,KC;Thompson,MM;Takeyasu,K;Schwartz,RJ;Owens,GK
通讯作者：
Owens,GK
培养的大鼠肾系膜细胞中胶原蛋白多态性。
DOI：
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发表时间：
1987
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Haralson,MA;Jacobson,HR;Hoover,RL
通讯作者：
Hoover,RL
小鼠血管平滑肌 α-肌动蛋白基因的 5 侧翼区域在功能启动子内包含进化保守的序列基序。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Min,BH;Foster,DN;Strauch,AR
通讯作者：
Strauch,AR