Improvement of base selectivity and binding affinity by controlling hydrogen bonding motifs between nucleobases and isoxanthopterin: application to the detection of T/C mutation.
Improvement of base selectivity and binding affinity by controlling hydrogen bonding motifs between nucleobases and isoxanthopterin: application to the detection of T/C mutation.
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DOI：
10.1016/j.bmcl.2007.04.033
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发表时间：
2007-07
影响因子：
2.7
通讯作者：
Burki Rajendar;Yusuke Sato;S. Nishizawa;N. Teramae
中科院分区：
文献类型：
--
作者：
Burki Rajendar;Yusuke Sato;S. Nishizawa;N. Teramae
异黄蝶呤(IX)†与胸腺嘧啶(T)和胞嘧啶(C)结合，与腺嘌呤和鸟嘌呤相比亲和力较强，但T对C的碱基选择性中等。为了提高T与C的结合亲和力和碱基选择性，在IX中引入了一个甲基，称为3-甲基异黄蝶呤(3-†)，由此可能会降低与C的结合亲和力。事实上，3-Mix与T的特异性结合比IX更强，并失去了与C的结合亲和力。3-Mix与T的结合能力的提高将适合于T相关SNP分型的实际应用。
At an abasic site in an oligo-DNA duplex, isoxanthopterin (IX)†can bind to thymine (T) and cytosine (C) with strong affinity compared to adenine and guanine, but the base selectivity for T against C is moderate. In order to improve both binding affinity and base selectivity for T against C, a methyl group is introduced to IX, which is known as 3-methyl isoxanthopterin (3-MIX),†by which binding affinity for C is expected to decrease. Indeed, 3-MIX specifically binds to T more strongly than IX and loses its binding affinity for C. The improved binding ability of 3-MIX for T would be suitable for the practical use in SNP typing related to T.