Bovine serum albumin recognition via thermosensitive molecular imprinted macroporous hydrogels prepared at two different temperatures.
Bovine serum albumin recognition via thermosensitive molecular imprinted macroporous hydrogels prepared at two different temperatures.
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DOI：
10.1016/j.aca.2012.02.020
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发表时间：
2012-04
影响因子：
6.2
通讯作者：
Dan Ran;Yuzhi Wang;Xiaopin Jia;Chan Nie
中科院分区：
文献类型：
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作者：
Dan Ran;Yuzhi Wang;Xiaopin Jia;Chan Nie
在两种不同温度（25°C和-20 °C）下，通过水溶液自由基交联共聚反应制备了由2-丙烯酰胺基-2-甲基丙磺酸（AMPS）、N-异丙基丙烯酰胺（NIPAm）和丙烯酰胺（AAm）组成的新型温敏分子印迹水凝胶。牛血清白蛋白（BSA，pI 4.9，MW 66.0kDa）用作模板蛋白。考察了外界温度刺激对凝胶亲和性的影响，并确定了最佳结合条件。通过Langmuir等温线测定了凝胶与模板蛋白之间特异性相互作用的吸附容量（Qmax）和缔合常数（K）。选择了几种不同分子量和等电点的参比蛋白质来研究水凝胶的选择性。结果表明，形状记忆效应和电荷效应是影响识别的主要因素。将该印迹水凝胶用于蛋白质混合物和真实的样品中的BSA的特异性吸附，证明了其潜在的选择性。
A novel temperature-sensitive molecular imprinted hydrogel composed of 2-acrylamido-2-methyl-propanosulfonic acid (AMPS), N-isopropylacrylamide (NIPAm) and acrylamide (AAm) has been prepared by free-radical cross-linking copolymerization in aqueous solution under two different temperatures (25°C and −20°C). Bovine serum albumin (BSA, pI 4.9, MW 66.0kDa) is used as the template protein. The influence of the external temperature stimuli on the affinity of the hydrogels was investigated, and the optimal binding conditions were tested. The adsorption capacity (Qmax) and association constant (K) for the specific interaction between the hydrogel and the template protein were determined by Langmuir isotherm plots. Several types of reference protein, which are different in molecular weights and isoelectric points were chosen to investigate the selectivity of the hydrogels. It was shown that the shape memory and the charge effect were the major factors for the recognition. This imprinted hydrogel was used to specifically adsorb the BSA from the protein mixture and real sample, which demonstrated its potential selectivity.