Molecular cloning and expression of a cryptochrome gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L
Molecular cloning and expression of a cryptochrome gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L
复制标题
南极微藻衣藻隐花基因 CiCRY-DASH1 的分子克隆和表达。
DOI：
10.1007/s12033-019-00225-y
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发表时间：
2020
影响因子：
2.6
通讯作者：
Miao J
中科院分区：
文献类型：
--
作者：
ZhangX;Zheng Z;He Y;Liu L;Qu C;Miao J
隐色素（CRYs）是一种黄素结合蛋白，能感知蓝光和近紫外光，参与生物体的光反应和生物钟调节。本研究从南极微藻Chlamydomonas sp. ICE-L中克隆了编码一种隐花蛋白的完整开放阅读框（ORF） CiCRY-DASH1 （GenBank ID MK392361）。实时荧光定量PCR结果显示，CiCRY-DASH1在5℃、盐度32‰时表达量最高。蓝、黄、红灯正调控CiCRY-DASH1。此外，CiCRY-DASH1对极端极日夜处理能产生正向反应，并表现出一定的昼夜节律，说明CiCRY-DASH1参与昼夜节律，其表达受昼夜节律调节。紫外- b辐射对CiCRY-DASH1的影响比紫外- a辐射更明显，说明紫外- b光对南极微藻的伤害明显。
Cryptochromes (CRYs) are flavin-binding proteins that sense blue and near-ultraviolet light and participate in the photoreactions of organisms and the regulation of biological clocks. In this study, the complete open reading frame (ORF) of CiCRY-DASH1 (GenBank ID MK392361), encoding one kind of cryptochrome, was cloned from the Antarctic microalga Chlamydomonas sp. ICE-L. The quantitative real-time PCR study showed that the CiCRY-DASH1 had the highest expression at 5 °C and salinity of 32‰. The CiCRY-DASH1 was positively regulated by blue, yellow, or red light. Moreover, the CiCRY-DASH1 can positively respond to extreme polar day and night treatment and exhibit a certain circadian rhythm, which indicated that CiCRY-DASH1 participated in the circadian clock and its expression was regulated by circadian rhythms. And the CiCRY-DASH1 was more noticeably affected by ultraviolet-B radiation than ultraviolet-A radiation, indicating ultraviolet-B light does obvious damage to Antarctic microalgae.